Chemiluminescence immunoassay in microemulsions

Alexander Kamyshny, Shlomo Magdassi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


A novel chemiluminescence immunoassay which combines microparticles and microemulsion is described. The method is based on a specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.6μm). Dissolving the particles in a microemulsion-forming mixture containing bis(2,4,6-trichlorophenyl) oxalate in toluene (oil phase) and H2O2 in Triton X-100 (surfactant)/2-butanol (cosurfactant)/water leads to chemiluminescence. The dependence of the chemiluminescence intensity on the concentrations of antigen, antibody and fluorescer, as well as the kinetics of the chemiluminescence decay, were studied. Copyright (C) 1998 Elsevier Science B.V.

Original languageAmerican English
Pages (from-to)249-254
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Issue number5
StatePublished - 31 Jul 1998


  • Adsorption
  • Chemiluminescence
  • IgG
  • Immunoassay
  • Microemulsions
  • Microparticles


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