Chemiluminescence immunoassay in microemulsions

Alexander Kamyshny; Shlomo Magdassi

doi:10.1016/S0927-7765(98)00044-7

Chemiluminescence immunoassay in microemulsions

Alexander Kamyshny, Shlomo Magdassi^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A novel chemiluminescence immunoassay which combines microparticles and microemulsion is described. The method is based on a specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.6μm). Dissolving the particles in a microemulsion-forming mixture containing bis(2,4,6-trichlorophenyl) oxalate in toluene (oil phase) and H₂O₂ in Triton X-100 (surfactant)/2-butanol (cosurfactant)/water leads to chemiluminescence. The dependence of the chemiluminescence intensity on the concentrations of antigen, antibody and fluorescer, as well as the kinetics of the chemiluminescence decay, were studied. Copyright (C) 1998 Elsevier Science B.V.

Original language	English
Pages (from-to)	249-254
Number of pages	6
Journal	Colloids and Surfaces B: Biointerfaces
Volume	11
Issue number	5
DOIs	https://doi.org/10.1016/S0927-7765(98)00044-7
State	Published - 31 Jul 1998

Keywords

Adsorption
Chemiluminescence
IgG
Immunoassay
Microemulsions
Microparticles

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10.1016/S0927-7765(98)00044-7

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AU - Magdassi, Shlomo

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AB - A novel chemiluminescence immunoassay which combines microparticles and microemulsion is described. The method is based on a specific interaction of an antibody with an antigen preadsorbed onto fluorescer (perylene) microparticles (mean diameter 0.8-1.6μm). Dissolving the particles in a microemulsion-forming mixture containing bis(2,4,6-trichlorophenyl) oxalate in toluene (oil phase) and H2O2 in Triton X-100 (surfactant)/2-butanol (cosurfactant)/water leads to chemiluminescence. The dependence of the chemiluminescence intensity on the concentrations of antigen, antibody and fluorescer, as well as the kinetics of the chemiluminescence decay, were studied. Copyright (C) 1998 Elsevier Science B.V.

KW - Adsorption

KW - Chemiluminescence

KW - IgG

KW - Immunoassay

KW - Microemulsions

KW - Microparticles

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JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 5

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Chemiluminescence immunoassay in microemulsions

Abstract

Keywords

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