Chicken red blood cells as a substrate for direct polymerase chain reaction

H. Khatib*, Y. Gruenbaum

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0·125 to 0·25 μl.

Original languageEnglish
Pages (from-to)53-54
Number of pages2
JournalAnimal Genetics
Volume27
Issue number1
DOIs
StatePublished - Feb 1996

Keywords

  • Blood
  • Chicken
  • Direct polymerase chain reaction
  • Red blood cells

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