Abstract
A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0·125 to 0·25 μl.
Original language | English |
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Pages (from-to) | 53-54 |
Number of pages | 2 |
Journal | Animal Genetics |
Volume | 27 |
Issue number | 1 |
DOIs | |
State | Published - Feb 1996 |
Keywords
- Blood
- Chicken
- Direct polymerase chain reaction
- Red blood cells