Chicken red blood cells as a substrate for direct polymerase chain reaction

H. Khatib; Y. Gruenbaum

doi:10.1111/j.1365-2052.1996.tb01177.x

Chicken red blood cells as a substrate for direct polymerase chain reaction

H. Khatib^*
, Y. Gruenbaum

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0·125 to 0·25 μl.

Original language	English
Pages (from-to)	53-54
Number of pages	2
Journal	Animal Genetics
Volume	27
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2052.1996.tb01177.x
State	Published - Feb 1996

Keywords

Blood
Chicken
Direct polymerase chain reaction
Red blood cells

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10.1111/j.1365-2052.1996.tb01177.x

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title = "Chicken red blood cells as a substrate for direct polymerase chain reaction",

abstract = "A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0·125 to 0·25 μl.",

keywords = "Blood, Chicken, Direct polymerase chain reaction, Red blood cells",

author = "H. Khatib and Y. Gruenbaum",

year = "1996",

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doi = "10.1111/j.1365-2052.1996.tb01177.x",

language = "אנגלית",

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AU - Khatib, H.

AU - Gruenbaum, Y.

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AB - A protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0·125 to 0·25 μl.

KW - Blood

KW - Chicken

KW - Direct polymerase chain reaction

KW - Red blood cells

UR - https://www.scopus.com/pages/publications/0030032538

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JO - Animal Genetics

JF - Animal Genetics

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Chicken red blood cells as a substrate for direct polymerase chain reaction

Abstract

Keywords

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