IL2-PE40 is a chimeric protein composed of human interleukin-2 (IL2) genetically fused to a modified form of Pseudomonas exotoxin lacking the cell recognition domain. IL2-PE40 is cytotoxic for IL2 receptor-bearing lymphocytes in culture and can inhibit activation of T cells in vivo. IL2-PE40 can significantly diminish antigen-stimulated proliferation of lymphocytes sensitized to myelin basic protein. Intraperitoneal administration of IL2-PE40 not only markedly inhibits the clinical manifestations of adoptively transferred relapsing experimental allergic encephalomyelitis but also dramatically reduces both inflammation and demyelination characteristic of the disease.
Bibliographical noteFunding Information:
This work was supported by VA Merit Review Funds and by Research Grant 1929-A-1 from the Multiple Sclerosis Society. We gratefully acknowledge the technical assistance of Craig Kamerath, Greg Gilson, Beckie McLain and Huschang Komeyli.
Supported by VA Merit Review and Research Grant 1929-A-1 from the National Multiple Sclerosis Society, U.S.A.
- Chimeric cytotoxin
- Experimental allergic encephalomyelitis
- Pseudomonas exotoxin