Chiral discrimination using an immunosensor

Oliver Hofstetter; Heike Hofstetter; Meir Wilchek; Volker Schurig; Bernard S. Green

doi:10.1038/7927

Chiral discrimination using an immunosensor

Oliver Hofstetter, Heike Hofstetter, Meir Wilchek, Volker Schurig, Bernard S. Green^*

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

107 Scopus citations

Abstract

Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized α- amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.

Original language	English
Pages (from-to)	371-374
Number of pages	4
Journal	Nature Biotechnology
Volume	17
Issue number	4
DOIs	https://doi.org/10.1038/7927
State	Published - Apr 1999

Keywords

Amino acid
Applied immunology
Chiral sensor

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10.1038/7927

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abstract = "Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized α- amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.",

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Chiral discrimination using an immunosensor

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