Chitinolytic activity of an endophytic strain of Bacillus cereus

S. Pleban; L. Chernin; I. Chet

doi:10.1046/j.1472-765X.1997.00224.x

Chitinolytic activity of an endophytic strain of Bacillus cereus

S. Pleban, L. Chernin^*, I. Chet

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. The enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (GlcNAc)₂ from the non-reducing end of trimeric chitin derivatives. The chitinase exhibited activity over the pH range 4.5-7.5 and was stable between pH 4.0 and 8.5. The enzyme had an isoelectric point of 6.4. Application of B. cereus 65 directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctonia solani.

Original language	English
Pages (from-to)	284-288
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1046/j.1472-765X.1997.00224.x
State	Published - 1997

Access to Document

10.1046/j.1472-765X.1997.00224.x

Cite this

@article{9a666bbe4eb84183b92379c9ec40928a,

title = "Chitinolytic activity of an endophytic strain of Bacillus cereus",

abstract = "Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. The enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (GlcNAc)2 from the non-reducing end of trimeric chitin derivatives. The chitinase exhibited activity over the pH range 4.5-7.5 and was stable between pH 4.0 and 8.5. The enzyme had an isoelectric point of 6.4. Application of B. cereus 65 directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctonia solani.",

author = "S. Pleban and L. Chernin and I. Chet",

year = "1997",

doi = "10.1046/j.1472-765X.1997.00224.x",

language = "אנגלית",

volume = "25",

pages = "284--288",

journal = "Letters in Applied Microbiology",

issn = "0266-8254",

publisher = "Oxford University Press",

number = "4",

}

TY - JOUR

T1 - Chitinolytic activity of an endophytic strain of Bacillus cereus

AU - Pleban, S.

AU - Chernin, L.

AU - Chet, I.

PY - 1997

Y1 - 1997

N2 - Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. The enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (GlcNAc)2 from the non-reducing end of trimeric chitin derivatives. The chitinase exhibited activity over the pH range 4.5-7.5 and was stable between pH 4.0 and 8.5. The enzyme had an isoelectric point of 6.4. Application of B. cereus 65 directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctonia solani.

AB - Bacillus cereus strain 65, previously isolated as an endophyte of Sinapis, was shown to produce and excrete a chitinase with an apparent molecular mass of 36 kDa. The enzyme was classified as a chitobiosidase because it was able to cleave diacetylchitobiose (GlcNAc)2 from the non-reducing end of trimeric chitin derivatives. The chitinase exhibited activity over the pH range 4.5-7.5 and was stable between pH 4.0 and 8.5. The enzyme had an isoelectric point of 6.4. Application of B. cereus 65 directly to soil significantly protected cotton seedlings from root rot disease caused by Rhizoctonia solani.

UR - http://www.scopus.com/inward/record.url?scp=0030770243&partnerID=8YFLogxK

U2 - 10.1046/j.1472-765X.1997.00224.x

DO - 10.1046/j.1472-765X.1997.00224.x

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 9351279

AN - SCOPUS:0030770243

SN - 0266-8254

VL - 25

SP - 284

EP - 288

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 4

ER -

Chitinolytic activity of an endophytic strain of Bacillus cereus

Abstract

Access to Document

Other files and links

Fingerprint

Cite this