TY - JOUR
T1 - Chorionic villus cDNA library displays expression of butyrylcholinesterase
T2 - Putative genetic disposition for ecological danger
AU - Zakut, Haim
AU - Lieman‐Hurwitz, Judith
AU - Zamir, Ronit
AU - Sindell, Lilian
AU - Ginzberg, Dalia
AU - Soreq, Hermona
PY - 1991/8
Y1 - 1991/8
N2 - Gene expression in chorionic villi may be particularly vulnerable to environmental exposure to poisonous substances. To reveal villus gene products which are thus subject to poisoning, molecular cloning was employed. A single sample of apparently normal chorionic villi (approximately 40 mg, from 9 weeks' gestation) was microscopically dissected to ensure purity of fetal tissue. Total RNA was extracted by isothiocyanate and directly employed for reverse transcription. A chorionic villus cDNA library was constructed from this preparation in the phage vector lambda gt10 and contained 60 000 independent recombinants. In the present study, this cDNA library was screened with labelled cDNA probes encoding human butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE). Nine BCHEcDNA clones were isolated out of 1.6 × 106 phages (5.7 × 10−6 of screened recombinants) and exhibited similar restriction patterns to those observed for BCHEcDNA from other human tissues. In contrast, no ACHEcDNA clones could be found in 4.0 × 106 screened phages (<2.5 × 10−6 of recombinants). These findings demonstrate efficient transcription (similar to fetal brain levels) from the BCHE gene but not from the ACHE gene in chorionic villi, and support the notion that BCHE is involved in chorionic villus growth and development.
AB - Gene expression in chorionic villi may be particularly vulnerable to environmental exposure to poisonous substances. To reveal villus gene products which are thus subject to poisoning, molecular cloning was employed. A single sample of apparently normal chorionic villi (approximately 40 mg, from 9 weeks' gestation) was microscopically dissected to ensure purity of fetal tissue. Total RNA was extracted by isothiocyanate and directly employed for reverse transcription. A chorionic villus cDNA library was constructed from this preparation in the phage vector lambda gt10 and contained 60 000 independent recombinants. In the present study, this cDNA library was screened with labelled cDNA probes encoding human butyrylcholinesterase (BCHE) and acetylcholinesterase (ACHE). Nine BCHEcDNA clones were isolated out of 1.6 × 106 phages (5.7 × 10−6 of screened recombinants) and exhibited similar restriction patterns to those observed for BCHEcDNA from other human tissues. In contrast, no ACHEcDNA clones could be found in 4.0 × 106 screened phages (<2.5 × 10−6 of recombinants). These findings demonstrate efficient transcription (similar to fetal brain levels) from the BCHE gene but not from the ACHE gene in chorionic villi, and support the notion that BCHE is involved in chorionic villus growth and development.
KW - Butyrylcholinesterase mRNA
KW - cDNA library
KW - Chorionic villi
KW - Organophosphorous poisoning
UR - http://www.scopus.com/inward/record.url?scp=0025744865&partnerID=8YFLogxK
U2 - 10.1002/pd.1970110817
DO - 10.1002/pd.1970110817
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C2 - 1722578
AN - SCOPUS:0025744865
SN - 0197-3851
VL - 11
SP - 597
EP - 607
JO - Prenatal Diagnosis
JF - Prenatal Diagnosis
IS - 8
ER -