CHox-cad characterization and rooster sperm preservation as a first step in the generation of transgenic chickens with modified homeobox genes

Yosef Gruenbaum, Ayala Frumkin, Zehava Rangini, Elie Revel, Sinai Yarus, Yael Margalit, Hasan Khatib, A Darvsi, A Fainsod

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

The characterization of a chicken homeobox gene that contains a homeobox sequence belonging to the cad family of homeodomains (the homeobox-translated region) is described. CHox-cad genomic and cDNA clones were isolated and analyzed. Northern and in situ hybridization analyses revealed that the CHox-cad gene probably encodes a single transcript of 2.6 kb that first appears in epiblastic cells when gastrulation begins, in the primitive streak, and in definitive endoderm. At later stages of development, CHox-cad is expressed in the endodermal lining of the developing gut. In addition, one target site of CHox-cad was identified. The idea of a possible role of homeobox genes in regulating quantitative traits (QTs) was examined in layer-cross and broiler populations, using CHox-cad as a probe. The results of this study revealed significant marker associations with several QTs. In order to further characterize CHox-cad regulation and the effects of its ectopic expression in the chicken, we have started to produce transgenic chickens using sperm cells as vectors. Some properties of the buffered semen diluent that is used in these experiments are described.
Original languageEnglish
Title of host publicationManipulation of the Avian Genome
EditorsRobert J Etches, Ann M Verrinder Gibbins
Place of PublicationBoca Raton
PublisherCRC Press, Taylor & Francis Group
Pages151-164
Number of pages14
Edition1st
ISBN (Electronic)9780203748282
ISBN (Print)0-8493-4216-3
StatePublished - 1993

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