@inbook{07cf37a1a1bb45e29539a932ca66ae45,
title = "Chromatin immunoprecipitation in mouse hippocampal cells and tissues",
abstract = "Chromatin immunoprecipitation (ChIP) has been developed for studying protein-DNA interactions and has been extensively used for mapping the localization of posttranslationally modified histones, histone variants, transcription factors, or chromatin modifying enzymes at a given locus or on a genome-wide scale. ChIP methods have been modified and improved over the years to fit a variety of different cell types and tissues. Here, we present a detailed protocol for hippocampal ChIP, of both minced tissue and enzyme-separated hippocampal cells. This protocol enables to study chromatin-protein interactions in a specified population of hippocampal cells, allowing to study chromatin regulation in the central nervous system in a variety of conditions and disorders. Our assay has been developed for histone modifications but is suited for any chromatin binding protein for which specific ChIP-grade antibodies are available.",
keywords = "Brain, ChIP, Chromatin, Chromatin immunoprecipitation, Hippocampus, Histone modifications, Neurons, Transcription",
author = "Sailaja, {Badi Sri} and Takumi Takizawa and Eran Meshorer",
year = "2012",
doi = "10.1007/978-1-61779-376-9_24",
language = "אנגלית",
isbn = "9781617793752",
series = "Methods in Molecular Biology",
pages = "353--364",
editor = "Ales Vancura",
booktitle = "Transcriptional Regulation",
}