Chromatin immunoprecipitation in mouse hippocampal cells and tissues

Badi Sri Sailaja*, Takumi Takizawa, Eran Meshorer

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

22 Scopus citations


Chromatin immunoprecipitation (ChIP) has been developed for studying protein-DNA interactions and has been extensively used for mapping the localization of posttranslationally modified histones, histone variants, transcription factors, or chromatin modifying enzymes at a given locus or on a genome-wide scale. ChIP methods have been modified and improved over the years to fit a variety of different cell types and tissues. Here, we present a detailed protocol for hippocampal ChIP, of both minced tissue and enzyme-separated hippocampal cells. This protocol enables to study chromatin-protein interactions in a specified population of hippocampal cells, allowing to study chromatin regulation in the central nervous system in a variety of conditions and disorders. Our assay has been developed for histone modifications but is suited for any chromatin binding protein for which specific ChIP-grade antibodies are available.

Original languageAmerican English
Title of host publicationTranscriptional Regulation
Subtitle of host publicationMethods and Protocols
EditorsAles Vancura
Number of pages12
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Brain
  • ChIP
  • Chromatin
  • Chromatin immunoprecipitation
  • Hippocampus
  • Histone modifications
  • Neurons
  • Transcription


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