Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA

Alon Goren; Fatih Ozsolak; Noam Shoresh; Manching Ku; Mazhar Adii; Chris Hart; Melissa Gymrek; Or Zuk; Aviv Regev; Patrice M. Milos; Bradley E. Bernstein

doi:10.1038/nmeth.1404

Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA

Alon Goren, Fatih Ozsolak, Noam Shoresh, Manching Ku, Mazhar Adii, Chris Hart, Melissa Gymrek, Or Zuk, Aviv Regev, Patrice M. Milos, Bradley E. Bernstein

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties, but current technologies Involve multiple steps that Introduce bias and Inefficiency. Here we apply a single-molecule approach to directly sequence chromatin lmmunopreclpltated DNA with minimal sample manipulation. This method Is compatible with just 50 pg of DNA and should thus facilitate charting chromatin maps from limited cell populations.

Original language	English
Pages (from-to)	47-49
Number of pages	3
Journal	Nature Methods
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.1404
State	Published - Jan 2010
Externally published	Yes

Bibliographical note

Funding Information:
We thank J. Robinson and members of the IGV platform for their help with data presentation. A.G. is supported by an EMBO long-term postdoctoral fellowship. M.K. is supported by a Croucher Foundation fellowship. A.R. is an investigator of the Merkin Foundation for Stem Cell Research at the Broad Institute. This research was supported by funds from the Burroughs Wellcome Fund (to B.E.B. and A.R.), Howard Hughes Medical Institute (to B.E.B. and A.R.), Partnership for Cures Culpeper Scholarship (to B.E.B.) and the US National Human Genome Research Institute.

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AU - Ozsolak, Fatih

AU - Shoresh, Noam

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AU - Adii, Mazhar

AU - Hart, Chris

AU - Gymrek, Melissa

AU - Zuk, Or

AU - Regev, Aviv

AU - Milos, Patrice M.

AU - Bernstein, Bradley E.

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Chromatin profiling by directly sequencing small quantities of immunoprecipitated DNA

Abstract

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