TY - JOUR
T1 - Chromatin state dynamics during blood formation
AU - Lara-Astiaso, David
AU - Weiner, Assaf
AU - Lorenzo-Vivas, Erika
AU - Zaretsky, Irina
AU - Jaitin, Diego Adhemar
AU - David, Eyal
AU - Keren-Shaul, Hadas
AU - Mildner, Alexander
AU - Winter, Deborah
AU - Jung, Steffen
AU - Friedman, Nir
AU - Amit, Ido
PY - 2014/8/22
Y1 - 2014/8/22
N2 - Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of homogeneous differentiation intermediates. We developed a high-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation. We identify 48,415 enhancer regions and characterize their dynamics.We find that lineage commitment involves de novo establishment of 17,035 lineage-specific enhancers. These enhancer repertoire expansions foreshadow transcriptional programs in differentiated cells. Combining our enhancer catalog with gene expression profiles, we elucidate the transcription factor network controlling chromatin dynamics and lineage specification in hematopoiesis. Together, our results provide a comprehensive model of chromatin dynamics during development.
AB - Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of homogeneous differentiation intermediates. We developed a high-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation. We identify 48,415 enhancer regions and characterize their dynamics.We find that lineage commitment involves de novo establishment of 17,035 lineage-specific enhancers. These enhancer repertoire expansions foreshadow transcriptional programs in differentiated cells. Combining our enhancer catalog with gene expression profiles, we elucidate the transcription factor network controlling chromatin dynamics and lineage specification in hematopoiesis. Together, our results provide a comprehensive model of chromatin dynamics during development.
UR - http://www.scopus.com/inward/record.url?scp=84907419194&partnerID=8YFLogxK
U2 - 10.1126/science.1256271
DO - 10.1126/science.1256271
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C2 - 25103404
AN - SCOPUS:84907419194
SN - 0036-8075
VL - 345
SP - 943
EP - 949
JO - Science
JF - Science
IS - 6199
ER -