Abstract
The metabolic products (non-volatile fatty acids) of 24 strains belonging to 4 Y. enterocolitica groups (typical, sucrose negative, rhamnose positive, melibiose and rhamnose positive) were examined by gas liquid chromatography. All strains produced lactic, pyruvic and succinic acids. This technique did not allow any classification for taxonomic purposes. The evolution of lactic and succinic acid production was monitored for 72 hr in shaken and non-shaken cultures. The concentration of both acids increased in the first 24 hr, then decreased and increased again after 38 hr. Shaken cultures produced a smaller amount of lactic acid than did non-shaken cultures. Only traces of succinic acid were detected in shaken cultures. The quantities of metabolic products of the 4 strains grown in 5 media were significantly different. These observations suggest that the composition of the medium, the bacterial growth, inoculum size, incubation technique and the incubation time are important factors in the metabolic production by aeroanaerobic bacteria. Only constant results in the face of such varying factors could be of taxonomic value.
| Translated title of the contribution | Gas liquid chromatography of non-volatile fatty acid produced by Yersinia enterocolitica |
|---|---|
| Original language | French |
| Pages (from-to) | 37-46 |
| Number of pages | 10 |
| Journal | Annales de Microbiologie |
| Volume | 130 A |
| Issue number | 1 |
| State | Published - 1979 |
| Externally published | Yes |