Chronic kisspeptin administration stimulated gonadal development in pre-pubertal male yellowtail kingfish (Seriola lalandi; Perciformes) during the breeding and non-breeding season

Josephine N. Nocillado, Yonathan Zohar, Jakob Biran, Berta Levavi-Sivan, Abigail Elizur*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The kisspeptin system is now accepted as a key regulator of vertebrate reproductive function, particularly the onset of puberty. In teleosts, the stimulatory effect of exogenous kisspeptins has been demonstrated mainly at the hypothalamic and pituitary levels of the reproductive axis, with very limited information pertaining to gonadal response. We determined the effect of chronic peripheral administration of the conserved kisspeptin decapeptides (YNLNSFGLRY or Kiss1-10; and FNFNPFGLRF or Kiss2-10) on gonadal development of pre-pubertal yellowtail kingfish (Seriola lalandi), a Perciform teleost, during the breeding and non-breeding season. We utilized slow-release implants to chronically deliver the synthesized peptides, which were based on the yellowtail kingfish kiss1 and kiss2 cDNA sequences that we isolated. The expression level of kiss2r and gnrh1 in the brain or hypothalamus did not vary between treated and control groups. Pituitary expression of fshβ and lhβ was upregulated only with Kiss1-10 treatment regardless of the season. Based on histological evidence, gonadal development was stimulated in male fish with either Kiss1-10 or Kiss2-10, with Kiss2-10 being more effective during the non-breeding period. Overall, our results suggest that kisspeptins modulate the early gonadal development of male yellowtail kingfish, however that may vary with the breeding season.

Original languageAmerican English
Pages (from-to)168-176
Number of pages9
JournalGeneral and Comparative Endocrinology
Volume191
DOIs
StatePublished - 15 Sep 2013

Bibliographical note

Funding Information:
This work was generously supported by the Australian Seafood CRC (grant number 2008/745 ), the Fisheries Research and Development Corporation, and the University of the Sunshine Coast. We are grateful to CleanSeas Tuna Pty Ltd, South Australia, who supplied the experimental fish and provided personnel support. We thank the Lincoln Marine Science Centre (LMSC) and South Australian Research Development Institute for sharing their fish culture facilities, Dr. Trent D’Antignana and Mr. Claudio Giordano from LMSC for logistical and technical assistance, and the following for their expert help during the experiments: Dr. Erin Bubner, Mark Thomas, Rafael Rodríguez, Alejandro Mechaly, Eran Yanowski and Angelico Madaro. We also acknowledge the technical assistance of Mr. Daniel Powell, USC, and the constructive discussions with Dr. Nilli Zmora.

Keywords

  • Breeding season
  • Kingfish
  • Kisspeptin
  • Puberty

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