Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

Lin Xu; Ying Yi Huo; Zheng Yang Li; Chun Sheng Wang; Aharon Oren; Xue Wei Xu

doi:10.1007/s10482-015-0390-x

Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

Lin Xu, Ying Yi Huo, Zheng Yang Li, Chun Sheng Wang, Aharon Oren, Xue Wei Xu^*

^*Corresponding author for this work

Department of Plant and Environmental Sciences (Natural Sciences)

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46^T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C_15:0, iso-C_17:0 3-OH, iso-C_17:1ω9c and summed feature 3 (comprising iso-C_15:0 2-OH and/or C_16:1ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46^T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46^T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacteriumtakakiae A1-2^T (99.1 %), C. taiwanense BCRC 17412^T (98.0 %), C. taeanense PHA3-4^T (97.3 %), C. hispalense DSM 25574^T (97.3 %), C. camelliae THG C4-1^T (97.2 %), C. gregarium DSM 19109^T (97.1 %) and C. wanjuense R2A10-2^T (97.0 %). The DNA–DNA relatedness values between strain DY46^T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46^T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46^T (=CGMCC 1.12663^T = JCM 19801^T).

Original language	American English
Pages (from-to)	979-989
Number of pages	11
Journal	Antonie van Leeuwenhoek
Volume	107
Issue number	4
DOIs	https://doi.org/10.1007/s10482-015-0390-x
State	Published - Apr 2015

Bibliographical note

Funding Information:
This work was supported by grants from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03 and DY125-14-E-02) and the National Natural Science Foundation of China (41276173).

Publisher Copyright:
© 2015, Springer International Publishing Switzerland.

Keywords

Chryseobacterium profundimari
Deep-sea sediment
Flavobacteriaceae
Polyphasic taxonomy
The Atlantic Ocean

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10.1007/s10482-015-0390-x

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title = " Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment",

abstract = "A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C15:0, iso-C17:0 3-OH, iso-C17:1ω9c and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacteriumtakakiae A1-2T (99.1 %), C. taiwanense BCRC 17412T (98.0 %), C. taeanense PHA3-4T (97.3 %), C. hispalense DSM 25574T (97.3 %), C. camelliae THG C4-1T (97.2 %), C. gregarium DSM 19109T (97.1 %) and C. wanjuense R2A10-2T (97.0 %). The DNA–DNA relatedness values between strain DY46T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46T (=CGMCC 1.12663T = JCM 19801T).",

keywords = "Chryseobacterium profundimari, Deep-sea sediment, Flavobacteriaceae, Polyphasic taxonomy, The Atlantic Ocean",

author = "Lin Xu and Huo, {Ying Yi} and Li, {Zheng Yang} and Wang, {Chun Sheng} and Aharon Oren and Xu, {Xue Wei}",

note = "Funding Information: This work was supported by grants from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03 and DY125-14-E-02) and the National Natural Science Foundation of China (41276173). Publisher Copyright: {\textcopyright} 2015, Springer International Publishing Switzerland.",

year = "2015",

month = apr,

doi = "10.1007/s10482-015-0390-x",

language = "American English",

volume = "107",

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journal = "Antonie van Leeuwenhoek",

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T1 - Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

AU - Xu, Lin

AU - Huo, Ying Yi

AU - Li, Zheng Yang

AU - Wang, Chun Sheng

AU - Oren, Aharon

AU - Xu, Xue Wei

N1 - Funding Information: This work was supported by grants from China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-R-03 and DY125-14-E-02) and the National Natural Science Foundation of China (41276173). Publisher Copyright: © 2015, Springer International Publishing Switzerland.

PY - 2015/4

Y1 - 2015/4

N2 - A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C15:0, iso-C17:0 3-OH, iso-C17:1ω9c and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacteriumtakakiae A1-2T (99.1 %), C. taiwanense BCRC 17412T (98.0 %), C. taeanense PHA3-4T (97.3 %), C. hispalense DSM 25574T (97.3 %), C. camelliae THG C4-1T (97.2 %), C. gregarium DSM 19109T (97.1 %) and C. wanjuense R2A10-2T (97.0 %). The DNA–DNA relatedness values between strain DY46T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46T (=CGMCC 1.12663T = JCM 19801T).

AB - A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C15:0, iso-C17:0 3-OH, iso-C17:1ω9c and summed feature 3 (comprising iso-C15:0 2-OH and/or C16:1ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacteriumtakakiae A1-2T (99.1 %), C. taiwanense BCRC 17412T (98.0 %), C. taeanense PHA3-4T (97.3 %), C. hispalense DSM 25574T (97.3 %), C. camelliae THG C4-1T (97.2 %), C. gregarium DSM 19109T (97.1 %) and C. wanjuense R2A10-2T (97.0 %). The DNA–DNA relatedness values between strain DY46T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46T (=CGMCC 1.12663T = JCM 19801T).

KW - Chryseobacterium profundimari

KW - Deep-sea sediment

KW - Flavobacteriaceae

KW - Polyphasic taxonomy

KW - The Atlantic Ocean

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DO - 10.1007/s10482-015-0390-x

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AN - SCOPUS:84925538706

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Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

Abstract

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Keywords

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