Chryseobacterium profundimaris sp. nov., a new member of the family Flavobacteriaceae isolated from deep-sea sediment

A Gram-stain negative, strictly aerobic, rod-shaped, non-motile bacterium, designated strain DY46^T, was isolated from Atlantic Ocean sediment. The isolate was found to grow in medium containing 0–3.0 % (w/v) NaCl (optimally at 0–1.0 %), at 4–37 °C and pH 5.0–8.0. Chemotaxonomic analysis detected MK-6 as the sole isoprenoid quinone. The major fatty acids were identified iso-C_15:0, iso-C_17:0 3-OH, iso-C_17:1ω9c and summed feature 3 (comprising iso-C_15:0 2-OH and/or C_16:1ω7c). The DNA G + C content was determined to be 40.7 mol %. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain DY46^T falls within the cluster comprising Chryseobacterium species. The levels of 16S rRNA gene sequence similarity between strain DY46^T and the type strains of the Chryseobacterium species with validly published names ranged from 92.4 to 99.1 %, the high values (>97 %) being with Chryseobacteriumtakakiae A1-2^T (99.1 %), C. taiwanense BCRC 17412^T (98.0 %), C. taeanense PHA3-4^T (97.3 %), C. hispalense DSM 25574^T (97.3 %), C. camelliae THG C4-1^T (97.2 %), C. gregarium DSM 19109^T (97.1 %) and C. wanjuense R2A10-2^T (97.0 %). The DNA–DNA relatedness values between strain DY46^T and the type strains of the above closely related species were 47, 57, 24, 34, 6, 40 and 21 %, respectively. On the basis of phenotypic and genotypic characteristics, strain DY46^T represents a novel member within the genus Chryseobacterium, for which the name Chryseobacterium profundimaris is proposed. The type strain is DY46^T (=CGMCC 1.12663^T = JCM 19801^T).