Cis -regulatory elements that confer differential expression upon the rat gene encoding phosphoenolpyruvate carboxykinase in kidney and liver

Tsipora Shoshani, Nissim Benvenisty, Michael Trus, Lea Reshef*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The PCK gene, encoding cytosolic phosphoenolpyruvate carboxykinase, is specifically expressed in gluconeogenic tissues, liver and kidney. Hence it serves as a model of a class of single-copy genes whose transcription is restricted to a few tissues, rather than a unique tissue. To begin delineating the mechanisms that govern this pattern of expression, cis-regulatory elements of PCK were examined using transient transfection assays in PCK-expressing kidney and hepatoma cell lines. The analyses enabled us to identify a proximal element, between nucleotide (nt) positions -121 and -98, relative to the transcription start point that is sufficient for specific expression in kidney cells, but is just one of the elements required for expression in hepatoma cells. A distal element (between nt -487 and -417), which is essential for hepatoma-specific expression, is not needed in kidney cells. We suggest that the differential regulation of PCK expression in the liver and kidney results from an interplay between different cis-regulatory elements and trans-acting factors.

Original languageAmerican English
Pages (from-to)279-283
Number of pages5
JournalGene
Volume101
Issue number2
DOIs
StatePublished - 30 May 1991

Bibliographical note

Funding Information:
We thank Hannah Cohen for technical assistance. This work was supported by BSF grant 88-00264a nd by a Basic Research Foundation grant administered by the Israel Academy of Sciences and Hum~ities.

Keywords

  • Cell specificity
  • DNA-mediated gene transfer
  • gene expression
  • hepatoma cells
  • kidney cells
  • nuclear factor-1
  • recombinant DNA

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