CKAMP44: A brain-specific protein attenuating short-term synaptic plasticity in the dentate gyrus

Jakob Von Engelhardt, Volker Mack, Rolf Sprengel, Netta Kavenstock, Ka Wan Li, Yael Stern-Bach, August B. Smit, Peter H. Seeburg, Hannah Monyer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

221 Scopus citations

Abstract

CKAMP44, identified here by a proteomic approach, is a brain-specific type I transmembrane protein that associates with AMPA receptors in synaptic spines. CKAMP44 expressed in Xenopus oocytes reduced GluA1- and A2-mediated steady-state currents, but did not affect kainate- or N-methyl-D-aspartate (NMDA) receptor-mediated currents. Mouse hippocampal CA1 pyramidal neurons expressed CKAMP44 at low abundance, and overexpression of CKAMP44 led to stronger and faster AMPA receptor desensitization, slower recovery from desensitization, and a reduction in the paired-pulse ratio of AMPA currents. By contrast, dentate gyrus granule cells exhibited strong CKAMP44 expression, and CKAMP44 knockout increased the paired-pulse ratio of AMPA currents in lateral and medial perforant path-granule cell synapses. CKAMP44 thus modulates short-term plasticity at specific excitatory synapses.

Original languageAmerican English
Pages (from-to)1518-1522
Number of pages5
JournalScience
Volume327
Issue number5972
DOIs
StatePublished - 19 Mar 2010

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