Clinical Phage Microbiology: a suggested framework and recommendations for the in-vitro matching steps of phage therapy

Daniel Gelman, Ortal Yerushalmy, Sivan Alkalay-Oren, Chani Rakov, Shira Ben-Porat, Leron Khalifa, Karen Adler, Mohanad Abdalrhman, Shunit Coppenhagen-Glazer, Saima Aslam, Robert T. Schooley, Ran Nir-Paz, Ronen Hazan*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

34 Scopus citations

Abstract

Phage therapy is a promising solution for bacterial infections that are not eradicated by conventional antibiotics. A crucial element of this approach is appropriate matching of bacteriophages and antibiotics to the bacterial target according to the clinical setting. However, there is currently little consistency in the protocols used for the laboratory evaluation of bacteriophages intended for antibacterial treatment. In this Personal View, we suggest a framework aimed to match appropriate bacteriophage-based treatments in clinical microbiology laboratories. This framework, which we have termed Clinical Phage Microbiology, is based on the current research on phage treatments. In addition, we discuss special cases that might require additional relevant evaluation, including bacteriophage interactions with the host immune response, biofilm-associated infections, and polymicrobial infections. The Clinical Phage Microbiology pipeline could serve as the basis for future standardisation of laboratory protocols for personalised phage therapy.

Original languageAmerican English
Pages (from-to)e555-e563
JournalThe Lancet Microbe
Volume2
Issue number10
DOIs
StatePublished - Oct 2021

Bibliographical note

Publisher Copyright:
© 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 license

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