TY - JOUR
T1 - Clinostomum complanatum and Clinostomum marginatum (Rudolphi, 1819) (Digenea: Clinostomidae) Are Separate Species Based on Differences in Ribosomal DNA
AU - Dzikowski, R.
AU - Levy, M. G.
AU - Poore, M. F.
AU - Flowers, J. R.
AU - Paperna, I.
PY - 2004/4
Y1 - 2004/4
N2 - Infections by metacercariae of Clinostomum (Leidy, 1856) species adversely affect aquacultured fish and are potentially transmissible to humans. Molecular methodologies are efficient tools, which enable diagnosis of all life-history stages of trematodes in their diverse hosts. The small subunit of ribosomal DNA genes of adults of the Old World Clinostomum complanatum (Rudolphi, 1819) and the New World Clinostomum marginatum (Rudolphi, 1819), obtained from a little egret Egretta garzetta (Linnaeus, 1766) and the great blue heron Ardea herodias (Linnaeus, 1758), respectively, were amplified, sequenced, and aligned. The resulting alignment was used to develop a genetic assay to differentiate between these species.
AB - Infections by metacercariae of Clinostomum (Leidy, 1856) species adversely affect aquacultured fish and are potentially transmissible to humans. Molecular methodologies are efficient tools, which enable diagnosis of all life-history stages of trematodes in their diverse hosts. The small subunit of ribosomal DNA genes of adults of the Old World Clinostomum complanatum (Rudolphi, 1819) and the New World Clinostomum marginatum (Rudolphi, 1819), obtained from a little egret Egretta garzetta (Linnaeus, 1766) and the great blue heron Ardea herodias (Linnaeus, 1758), respectively, were amplified, sequenced, and aligned. The resulting alignment was used to develop a genetic assay to differentiate between these species.
UR - http://www.scopus.com/inward/record.url?scp=2442651905&partnerID=8YFLogxK
U2 - 10.1645/GE-159R
DO - 10.1645/GE-159R
M3 - Article
C2 - 15165071
AN - SCOPUS:2442651905
SN - 0022-3395
VL - 90
SP - 413
EP - 414
JO - Journal of Parasitology
JF - Journal of Parasitology
IS - 2
ER -