Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Daniel Chamovitz; Iris Pecker; Gerhard Sandmann; Peter Böger; Joseph Hirschberg

doi:10.1515/znc-1990-0531

Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Daniel Chamovitz, Iris Pecker, Gerhard Sandmann, Peter Böger, Joseph Hirschberg

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

Original language	English
Pages (from-to)	482-486
Number of pages	5
Journal	Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume	45
Issue number	5
DOIs	https://doi.org/10.1515/znc-1990-0531
State	Published - May 1990

Keywords

Cyanobacteria
Gene Cloning
Herbicide Resistance
Norflurazon
Phytoene Dehydrogenase (= Phytoene Desaturase)

Access to Document

10.1515/znc-1990-0531

Cite this

@article{cb4820a39f68487495d43123c7d3cf44,

title = "Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria",

abstract = "The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.",

keywords = "Cyanobacteria, Gene Cloning, Herbicide Resistance, Norflurazon, Phytoene Dehydrogenase (= Phytoene Desaturase)",

author = "Daniel Chamovitz and Iris Pecker and Gerhard Sandmann and Peter B{\"o}ger and Joseph Hirschberg",

year = "1990",

month = may,

doi = "10.1515/znc-1990-0531",

language = "אנגלית",

volume = "45",

pages = "482--486",

journal = "Zeitschrift fur Naturforschung - Section C Journal of Biosciences",

issn = "0939-5075",

publisher = "Walter de Gruyter GmbH",

number = "5",

}

TY - JOUR

T1 - Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

AU - Chamovitz, Daniel

AU - Pecker, Iris

AU - Sandmann, Gerhard

AU - Böger, Peter

AU - Hirschberg, Joseph

PY - 1990/5

Y1 - 1990/5

N2 - The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

AB - The herbicide norflurazon inhibits carotene biosynthesis in photosynthetic organisms by blocking the enzyme phytoene dehydrogenase (= phytoene desaturase). We have isolated norflurazon-resistant mutants of the cyanobacterium Synechococcus PCC7942. The herbicide-resistance gene from the mutant NFZ4 has been cloned by genetic complementation of the resistance trait in wild type cells. The experiment described here illustrates the usefulness of employing cyanobacteria to clone herbicide-resistance genes in a quick and simple way.

KW - Cyanobacteria

KW - Gene Cloning

KW - Herbicide Resistance

KW - Norflurazon

KW - Phytoene Dehydrogenase (= Phytoene Desaturase)

UR - http://www.scopus.com/inward/record.url?scp=0025428194&partnerID=8YFLogxK

U2 - 10.1515/znc-1990-0531

DO - 10.1515/znc-1990-0531

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 2116131

AN - SCOPUS:0025428194

SN - 0939-5075

VL - 45

SP - 482

EP - 486

JO - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

JF - Zeitschrift fur Naturforschung - Section C Journal of Biosciences

IS - 5

ER -

Cloning a Gene Coding for Norflurazon Resistance in Cyanobacteria

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this