Cloning and expression of Bacillus thuringiensis israelensis δ-endotoxin DNA in B. sphaericus

E. Bar*, J. Lieman-Hurwitz, E. Rahamim, A. Keynan, N. Sandler

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Bacillus thuringiensis israelensisδ-endotoxin genes were cloned into Bacillus sphaericus 2362, producing stable transformants reacting with antibody to the 28- and 65-kDa B. thuringiensis israelensis crystal proteins and approximately 10 times more toxic to Aedes mosquito larvae than the original host strain. The LC50 after 48 hr of exposure of Aedes larvae to the most active transformed clone was 0.19 μg/ml, compared with an LC50 of 1.9 μg/ml for B. sphaericus 2362 and less than 0.1 μg/ml for B. thuringiensis israelensis. The cloning vector, plasmid pPL603E, was also effective in transforming B. subtilis 1E20 with B. thuringiensis israelensis DNA, producing highly toxic clones with less stable gene expression than the clones of B. sphaericus.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalJournal of Invertebrate Pathology
Volume57
Issue number2
DOIs
StatePublished - Mar 1991

Keywords

  • Aedes aegypti
  • Bacillus sphaericus
  • Bacillus subtilis
  • Bacillus thuringiensis
  • biological control
  • endotoxin

Fingerprint

Dive into the research topics of 'Cloning and expression of Bacillus thuringiensis israelensis δ-endotoxin DNA in B. sphaericus'. Together they form a unique fingerprint.

Cite this