Cloning and mapping of the potato virus Y genome and its in vitro expression

Miri Karchi; Ilan Sela; Orit Edelbaum; Assaf Ori; Benjamin Raccah; Arie Rosner; Yehuda Stram

doi:10.1007/BF00265631

Cloning and mapping of the potato virus Y genome and its in vitro expression

Miri Karchi, Ilan Sela^*, Orit Edelbaum, Assaf Ori, Benjamin Raccah, Arie Rosner, Yehuda Stram

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

Original language	English
Pages (from-to)	215-224
Number of pages	10
Journal	Virus Genes
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF00265631
State	Published - Sep 1990

Keywords

PVY
cloning
expression
viruses

Access to Document

10.1007/BF00265631

Cite this

@article{01791f6fea4041de99f89ad305f53b3a,

title = "Cloning and mapping of the potato virus Y genome and its in vitro expression",

abstract = "Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.",

keywords = "PVY, cloning, expression, viruses",

author = "Miri Karchi and Ilan Sela and Orit Edelbaum and Assaf Ori and Benjamin Raccah and Arie Rosner and Yehuda Stram",

year = "1990",

month = sep,

doi = "10.1007/BF00265631",

language = "אנגלית",

volume = "4",

pages = "215--224",

journal = "Virus Genes",

issn = "0920-8569",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Cloning and mapping of the potato virus Y genome and its in vitro expression

AU - Karchi, Miri

AU - Sela, Ilan

AU - Edelbaum, Orit

AU - Ori, Assaf

AU - Raccah, Benjamin

AU - Rosner, Arie

AU - Stram, Yehuda

PY - 1990/9

Y1 - 1990/9

N2 - Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

AB - Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

KW - PVY

KW - cloning

KW - expression

KW - viruses

UR - http://www.scopus.com/inward/record.url?scp=0025048370&partnerID=8YFLogxK

U2 - 10.1007/BF00265631

DO - 10.1007/BF00265631

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 1980175

AN - SCOPUS:0025048370

SN - 0920-8569

VL - 4

SP - 215

EP - 224

JO - Virus Genes

JF - Virus Genes

IS - 3

ER -

Cloning and mapping of the potato virus Y genome and its in vitro expression

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this