Cloning and mapping of the potato virus Y genome and its in vitro expression

Miri Karchi; Ilan Sela; Orit Edelbaum; Assaf Ori; Benjamin Raccah; Arie Rosner; Yehuda Stram

doi:10.1007/BF00265631

Cloning and mapping of the potato virus Y genome and its in vitro expression

Miri Karchi
, Ilan Sela^*
, Orit Edelbaum
, Assaf Ori
, Benjamin Raccah
, Arie Rosner
, Yehuda Stram

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

Original language	English
Pages (from-to)	215-224
Number of pages	10
Journal	Virus Genes
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF00265631
State	Published - Sep 1990

Keywords

PVY
cloning
expression
viruses

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10.1007/BF00265631

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abstract = "Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.",

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AU - Karchi, Miri

AU - Sela, Ilan

AU - Edelbaum, Orit

AU - Ori, Assaf

AU - Raccah, Benjamin

AU - Rosner, Arie

AU - Stram, Yehuda

PY - 1990/9

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N2 - Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

AB - Full-length cDNA of genomic RNA of potato virus Y (PVY) was cloned in one piece into a lambda vector. The order of the EcoRI and SalI fragments of the inserted cDNA was determined. This is the first report of the cloning of a long, expressible, potyvirus genome. The availability of such a clone is a prerequisite for any further study of the molecular biology of this group of viruses, as they are expressed into a self-processed primary polyprotein.

KW - PVY

KW - cloning

KW - expression

KW - viruses

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Cloning and mapping of the potato virus Y genome and its in vitro expression

Abstract

Keywords

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