Cloning and restriction analysis of ribosomal RNA genes from Mycobacterium smegmatis

H. Bercovier*, O. Kafri, D. Kornitzer, S. Sela

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

A genomic library of Mycobacterium smegmatis DNA was constructed in phage EMBL3. A clone (λ HB85) containing rRNA genes was isolated using as probes, fragments of E. coli rRNA cistron B. This cloned DNA fragment was mapped by restriction analysis and was shown to contain one complete set of rRNA genes (rRNA A). The physical mapping of the second set of rRNA genes of M. smegmatis (rRNA B) was done by restriction analysis of total chromosomal DNA. The two sets of rRNA genes showed highly conserved restriction sites within the respective sets but not in the flanking regions. The two rRNA sets of genes are organised like in the other eubacteria in the order 16S-23S-5S.

Original languageEnglish
Pages (from-to)125-128
Number of pages4
JournalFEMS Microbiology Letters
Volume57
Issue number2
DOIs
StatePublished - 15 Jan 1989

Keywords

  • Mycobacterium
  • Recombinant DNA
  • Restriction map
  • Ribosomal RNA genes

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