Cloning, expression, characterization, and nucleophile identification of family 3, Aspergillus niger β-glucosidase

Keyphrases

• Glycosidase 100%
• Complementary DNA (cDNA) 100%
• Cloning Expression 100%
• Glucosidase 100%
• Nucleophile 100%
• Aspergillus Niger 100%
• Expression Characterization 100%
• Quantitative PCR 50%
• Saccharomyces Cerevisiae 50%
• Genomic Library 50%
• Incubation 50%
• Sequence Analysis 50%
• Tandem Mass Spectrometry 50%
• Labeled Peptide 50%
• Pichia Pastoris 50%
• NMR Analysis 50%
• Kinetic Parameters 50%
• Fluoro 50%
• Anomeric Configuration 50%
• Electrospray Ionization 50%
• N-terminal Sequence 50%
• K(I) 50%
• Ultra-high Performance Liquid Chromatography (UHPLC) 50%
• Eluate 50%
• Fluoride 50%
• Glucosyl 50%
• Sequence Determination 50%
• Neutral Loss 50%
• Transglycosylation 50%
• Glycosyl-enzyme Intermediate 50%
• Peptic Digestion 50%
• Aspergillus Wentii 50%
• Aspartic Acid Residue 50%
• Triple Quadrupole Mass Spectrometry 50%
• Linamarin 50%
• Conduritol B Epoxide 50%
• Genomic Gene 50%
• Scanning Mode 50%

Immunology and Microbiology

• Aspergillus Niger 100%
• Peptides 100%
• Glucosidase 100%
• Glycoside Hydrolase 100%
• Amino Terminal Sequence 50%
• Saccharomyces cerevisiae 50%
• Gene Library 50%
• Liquid Chromatography 50%
• Sequence Analysis 50%
• Komagataella pastoris 50%
• Tandem Mass Spectrometry 50%
• Glycosylation 50%
• Electrospray 50%
• Kinetic Parameter 50%
• Aspergillus wentii 50%
• Quadrupole Mass Spectrometry 50%
• Reverse Transcription Polymerase Chain Reaction 50%
• Aspartic Acid 50%

Biochemistry, Genetics and Molecular Biology

• Enzyme 100%
• Aspergillus Niger 100%
• Glucosidase 100%
• Shotgun Sequencing 28%
• N-Terminus 14%
• Gene Library 14%
• Saccharomyces cerevisiae 14%
• Enzymatic Hydrolysis 14%
• High-Performance Liquid Chromatography 14%
• Glycoside Hydrolase 14%
• Glycosylation 14%
• Glycoside Hydrolase 14%
• Protein Sequencing 14%
• Glycosyl 14%
• Komagataella pastoris 14%
• Kinetic Parameter 14%
• Tandem Mass Spectrometry 14%
• Electrospray Ionization 14%
• Linamarin 14%
• Quadrupole Mass Spectrometry 14%
• Aspergillus wentii 14%
• Genomics 14%
• Aspartic Acid 14%
• Reverse Transcription Polymerase Chain Reaction 14%