Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division?

Heather I. McKhann; Florian Frugier; György Petrovics; Teodoro Coba De La Peña; Edouard Jurkevitch; Spencer Brown; Eva Kondorosi; Adam Kondorosi; Martin Crespi

doi:10.1023/A:1005899410389

Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division?

Heather I. McKhann, Florian Frugier, György Petrovics, Teodoro Coba De La Peña, Edouard Jurkevitch, Spencer Brown, Eva Kondorosi, Adam Kondorosi^*, Martin Crespi

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows horology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

Original language	American English
Pages (from-to)	771-780
Number of pages	10
Journal	Plant Molecular Biology
Volume	34
Issue number	5
DOIs	https://doi.org/10.1023/A:1005899410389
State	Published - Jul 1997

Bibliographical note

Funding Information:
The authors wish to thank Petra Bauer for providing the RNA samples from hormone-treated roots. Jacob Seel-er is gratefully acknowledged for his aid in computer-related matters, including protein structure predictions. H.I.M. was supported by a National Science Foundation (USA) post-doctoral fellowship in Plant Science, F.F. was the recipient of a fellowship of the Min-istère franc¸ais de l’Enseignement Superieur et de la Recherche, G.P. was supported within the framework of the Jumelage Program between the Gif and Szeged laboratories, T.C. was the recipient of a fellowship of the Caixa Galicia Foundation, Spain, and E.J. was the recipient of an EMBO long-term postdoctoral fellowship.

Keywords

Alfalfa
Cytokinin
G-protein β-subunit
RACK1 subfamily
WD-repeat proteins

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@article{7e61e4d00aa64462b25d25143096b523,

title = "Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division?",

abstract = "Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows horology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.",

keywords = "Alfalfa, Cytokinin, G-protein β-subunit, RACK1 subfamily, WD-repeat proteins",

author = "McKhann, {Heather I.} and Florian Frugier and Gy{\"o}rgy Petrovics and {Coba De La Pe{\~n}a}, Teodoro and Edouard Jurkevitch and Spencer Brown and Eva Kondorosi and Adam Kondorosi and Martin Crespi",

note = "Funding Information: The authors wish to thank Petra Bauer for providing the RNA samples from hormone-treated roots. Jacob Seel-er is gratefully acknowledged for his aid in computer-related matters, including protein structure predictions. H.I.M. was supported by a National Science Foundation (USA) post-doctoral fellowship in Plant Science, F.F. was the recipient of a fellowship of the Min-ist{\`e}re franc¸ais de l{\textquoteright}Enseignement Superieur et de la Recherche, G.P. was supported within the framework of the Jumelage Program between the Gif and Szeged laboratories, T.C. was the recipient of a fellowship of the Caixa Galicia Foundation, Spain, and E.J. was the recipient of an EMBO long-term postdoctoral fellowship.",

year = "1997",

month = jul,

doi = "10.1023/A:1005899410389",

language = "American English",

volume = "34",

pages = "771--780",

journal = "Plant Molecular Biology",

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T1 - Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa)

T2 - A role in hormone-mediated cell division?

AU - McKhann, Heather I.

AU - Frugier, Florian

AU - Petrovics, György

AU - Coba De La Peña, Teodoro

AU - Jurkevitch, Edouard

AU - Brown, Spencer

AU - Kondorosi, Eva

AU - Kondorosi, Adam

AU - Crespi, Martin

N1 - Funding Information: The authors wish to thank Petra Bauer for providing the RNA samples from hormone-treated roots. Jacob Seel-er is gratefully acknowledged for his aid in computer-related matters, including protein structure predictions. H.I.M. was supported by a National Science Foundation (USA) post-doctoral fellowship in Plant Science, F.F. was the recipient of a fellowship of the Min-istère franc¸ais de l’Enseignement Superieur et de la Recherche, G.P. was supported within the framework of the Jumelage Program between the Gif and Szeged laboratories, T.C. was the recipient of a fellowship of the Caixa Galicia Foundation, Spain, and E.J. was the recipient of an EMBO long-term postdoctoral fellowship.

PY - 1997/7

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N2 - Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows horology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

AB - Rhizobium meliloti can interact symbiotically with Medicago plants thereby inducing the formation of root nodules. Screening of a young nodule cDNA library led to the isolation of a cDNA from Medicago sativa, Msgbl, that comprises a new member of the RACK1 (Receptor of Activated C Kinase) subfamily of WD-repeat proteins. This subfamily shows horology to the β-subunit of heterotrimeric G proteins. Besides RACK1, this subfamily contains several plant genes including the well characterized auxin-inducible ArcA of tobacco. The Msgbl gene is strongly expressed in young embryos and in leaves and is induced upon cytokinin treatment of roots. Whereas northern analysis failed to reveal differences in expression between total RNA from roots and nodules, in situ hybridization demonstrated that the transcript was most abundant in dividing cells of nodule primordia and in the nodule meristem. Msgbl may be related to the signal transduction acting in response to hormone-mediated cell division.

KW - Alfalfa

KW - Cytokinin

KW - G-protein β-subunit

KW - RACK1 subfamily

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DO - 10.1023/A:1005899410389

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AN - SCOPUS:0031193616

SN - 0167-4412

VL - 34

SP - 771

EP - 780

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 5

ER -

Cloning of a WD-repeat-containing gene from alfalfa (Medicago sativa): A role in hormone-mediated cell division?

Abstract

Bibliographical note

Keywords

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