Cloning of bovine growth hormone gene and its expression in bacteria

E. Keshet*, A. Rosner, Y. Bernstein, M. Gorecki, H. Aviv

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A hybrid plasmid was constructed containing β-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the β-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused β-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH Unlike β-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.

Original languageEnglish
Pages (from-to)19-30
Number of pages12
JournalNucleic Acids Research
Volume9
Issue number1
DOIs
StatePublished - 10 Jan 1981
Externally publishedYes

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