Cloning of bovine growth hormone gene and its expression in bacteria

A hybrid plasmid was constructed containing β-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cloned DNA sequences are inserted into the β-lactamase gene in the correct reading frame for BGH synthesis. The hybrid gene is expressed in bacteria and the product, a fused β-lactamase-bovine growth hormone protein, is specifically immunoprecipitated with anti-serum to BGH Unlike β-lactamase, very little growth hormone containing sequences can be detected in the periplasmic space.