Cloning of Clostridium cellulovorans endo-1,4-β-glucanase genes

Oded Shoseyov*, Tetsuo Hamamoto, Frances Foong, Roy H. Doi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


A Clostridium cellulovorans lambda gt1l gene bank was screened for endo-1,4-β-glucanase [EC, EGase, Carboxy Methyl Cellulase (CMCase)] genes using a chromogenic substrate. Three genes (engA, engB, and engC) were isolated. The engB expressed the most active CMCase. The engA encoded a bifunctional enzyme that displayed endo-1,4-β-glucanase and β-glucosidase activities. The three recombinant glucanases, when expressed in Escherichia coli, were partially degraded into multiform active enzymes as evidenced by their SDS-PAGE-CMC zymograms. None of the clones could degrade crystalline cellulose, thus supporting the hypothesis that the integrity of the C. cellulovorans cellulase complex was essential for its 'true cellulase' activity.

Original languageAmerican English
Pages (from-to)667-672
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - 15 Jun 1990
Externally publishedYes

Bibliographical note

Funding Information:
ACKNOWLEDGMENWTSe: thank Reinhold Bruckner for his suggestions on the cloning strategy. O.S. was supported by fellowship SI-0057-87 from The United State-Israel Binational Agricultural Research and Development Fund. The research was supported in part by Department of Energy grant #DE-FG03-87FR13705( RHD).


Dive into the research topics of 'Cloning of Clostridium cellulovorans endo-1,4-β-glucanase genes'. Together they form a unique fingerprint.

Cite this