Cloning of the gene coding for chitobiase of Serratia marcescens

Hadar Kless; Yaron Sitrit; Ilan Chet; Amos B. Oppenheim

doi:10.1007/BF02464919

Cloning of the gene coding for chitobiase of Serratia marcescens

Hadar Kless, Yaron Sitrit, Ilan Chet, Amos B. Oppenheim^*

^*Corresponding author for this work

Department of Plant Pathology and Microbiology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.

Original language	English
Pages (from-to)	471-473
Number of pages	3
Journal	Zeitschrift fur Vererbungslehre
Volume	217
Issue number	2-3
DOIs	https://doi.org/10.1007/BF02464919
State	Published - Jun 1989

Keywords

Chitobiase
Serratia marcescens

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10.1007/BF02464919

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title = "Cloning of the gene coding for chitobiase of Serratia marcescens",

abstract = "A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.",

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AU - Chet, Ilan

AU - Oppenheim, Amos B.

PY - 1989/6

Y1 - 1989/6

N2 - A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.

AB - A λ phage DNA library of Serratia marcescens was constructed and a clone carrying the gene coding for chitobiase (E.C.3.2.1.29) was isolated and characterized. Deletion analysis limited the cloned region to 4.5 kb that is capable of efficient expression of chitobiase. Escherichia coli cells harboring a plasmid carrying the cloned gene express chitobiase constitutively. The molecular weight of the protein is about 95000 daltons. In exponentially growing E. coli cells the chitobiase enzyme was found to be secreted into the periplasm.

KW - Chitobiase

KW - Serratia marcescens

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Cloning of the gene coding for chitobiase of Serratia marcescens

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