CNPY4 inhibits the Hedgehog pathway by modulating membrane sterol lipids

Megan Lo, Amnon Sharir, Michael D. Paul, Hayarpi Torosyan, Christopher Agnew, Amy Li, Cynthia Neben, Pauline Marangoni, Libin Xu, David R. Raleigh, Natalia Jura*, Ophir D. Klein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The Hedgehog (HH) pathway is critical for development and adult tissue homeostasis. Aberrant HH signaling can lead to congenital malformations and diseases including cancer. Although cholesterol and several oxysterol lipids have been shown to play crucial roles in HH activation, the molecular mechanisms governing their regulation remain unresolved. Here, we identify Canopy4 (CNPY4), a Saposin-like protein, as a regulator of the HH pathway that modulates levels of membrane sterol lipids. Cnpy4–/– embryos exhibit multiple defects consistent with HH signaling perturbations, most notably changes in digit number. Knockdown of Cnpy4 hyperactivates the HH pathway in vitro and elevates membrane levels of accessible sterol lipids, such as cholesterol, an endogenous ligand involved in HH activation. Our data demonstrate that CNPY4 is a negative regulator that fine-tunes HH signal transduction, revealing a previously undescribed facet of HH pathway regulation that operates through control of membrane composition.

Original languageAmerican English
Article number2407
JournalNature Communications
Volume13
Issue number1
DOIs
StatePublished - 3 May 2022

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© 2022, The Author(s).

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