During a survey for Bartonella species in rodents in Vom, Plateau State, Nigeria, a brown rat (Rattus norvegicus) with multiple subcutaneous nodules was captured. Gross pathology examination revealed multifocal pale areas in the spleen and liver. Histopathologic examination of the liver revealed numerous multifocal well demarcated and walled-of granulomas compressing and destroying the hepatic cord and parenchyma. Granulomas contained Capillaria hepatica nematodes in different developmental stages with numerous typical ova. Tere was degeneration and necrosis of hepatocytes, bridging fibrosis and biliary hyperplasia and reduplication, surrounded by inflammatory foci composed of lymphocytes, plasma cells and macrophages. Tiny smooth bacterial colonies with metallic sheen were isolated from blood of the rat cultured on blood chocolate agar. DNA extracted from the blood and spleen of the rat was found positive for Bartonella spp. citrate synthase gene (gltA) by PCR. Sequence analysis shows 99% similarity to B. elizabethae. Te identification of two zoonotic agents in a rat highlights the importance of rats in the transmission cycle of zoonotic diseases in Nigeria.
|Original language||American English|
|Number of pages||4|
|Journal||Israel Journal of Veterinary Medicine|
|State||Published - Dec 2013|
- Bartonella elizabethae
- Capillaria hepatica
- Rattus norvegicus