Coated cross-species antibodies by mannosamine-biotin adduct confer protection against snake venom without eliciting humoral immune response

Tal Gefen; Jacob Pitcovski; Jacob Vaya; Soliman Khatib; Simi Krispel; E. Dan Heller; Elena Gaberman; Raphael Gorodetsky; Elina Aizenshtein

doi:10.1016/j.vaccine.2010.09.032

Coated cross-species antibodies by mannosamine-biotin adduct confer protection against snake venom without eliciting humoral immune response

Tal Gefen, Jacob Pitcovski, Jacob Vaya, Soliman Khatib, Simi Krispel, E. Dan Heller, Elena Gaberman, Raphael Gorodetsky, Elina Aizenshtein^*

^*Corresponding author for this work

Department of Animal Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Passive immunization with cross-species antibodies triggers the patient's immune response, thereby preventing repeated treatment. Mannosamine-biotin adduct (MBA) has been described as a masking agent for immunogenic reduction and here, the immunogenicity and biological activity of MBA-coated horse anti-viper venom (hsIgG) were compared to those of uncoated or PEGylated hsIgG. In in vitro tests, hsIgG binding was not affected by MBA conjugation. The immune response to hsIgG-MBA was about 8-fold and 32-fold lower than to PEG-coated and uncoated hsIgG, respectively. In vivo, hsIgG-MBA showed efficient venom-neutralization activity. We thus demonstrate the feasibility of using MBA as a masking agent for passive immunization with cross-species antibodies.

Original language	English
Pages (from-to)	8197-8202
Number of pages	6
Journal	Vaccine
Volume	28
Issue number	51
DOIs	https://doi.org/10.1016/j.vaccine.2010.09.032
State	Published - 29 Nov 2010

Keywords

Antigenicity reduction
Coated antibody
Mannosamine-biotin adduct
Passive vaccination
Snake venom

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10.1016/j.vaccine.2010.09.032

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title = "Coated cross-species antibodies by mannosamine-biotin adduct confer protection against snake venom without eliciting humoral immune response",

abstract = "Passive immunization with cross-species antibodies triggers the patient's immune response, thereby preventing repeated treatment. Mannosamine-biotin adduct (MBA) has been described as a masking agent for immunogenic reduction and here, the immunogenicity and biological activity of MBA-coated horse anti-viper venom (hsIgG) were compared to those of uncoated or PEGylated hsIgG. In in vitro tests, hsIgG binding was not affected by MBA conjugation. The immune response to hsIgG-MBA was about 8-fold and 32-fold lower than to PEG-coated and uncoated hsIgG, respectively. In vivo, hsIgG-MBA showed efficient venom-neutralization activity. We thus demonstrate the feasibility of using MBA as a masking agent for passive immunization with cross-species antibodies.",

keywords = "Antigenicity reduction, Coated antibody, Mannosamine-biotin adduct, Passive vaccination, Snake venom",

author = "Tal Gefen and Jacob Pitcovski and Jacob Vaya and Soliman Khatib and Simi Krispel and Heller, {E. Dan} and Elena Gaberman and Raphael Gorodetsky and Elina Aizenshtein",

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doi = "10.1016/j.vaccine.2010.09.032",

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AU - Gefen, Tal

AU - Pitcovski, Jacob

AU - Vaya, Jacob

AU - Khatib, Soliman

AU - Krispel, Simi

AU - Heller, E. Dan

AU - Gaberman, Elena

AU - Gorodetsky, Raphael

AU - Aizenshtein, Elina

PY - 2010/11/29

Y1 - 2010/11/29

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KW - Antigenicity reduction

KW - Coated antibody

KW - Mannosamine-biotin adduct

KW - Passive vaccination

KW - Snake venom

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JO - Vaccine

JF - Vaccine

IS - 51

ER -

Coated cross-species antibodies by mannosamine-biotin adduct confer protection against snake venom without eliciting humoral immune response

Abstract

Keywords

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