Coculture of mast cells with psoriatic fibroblasts: an experimental system for studying the two cell interactions

Francesca Levi-Schaffer; Laurent Klapholz; Louis Weinrauch; Meir Shalit

doi:10.1016/0926-9959(95)00050-N

Coculture of mast cells with psoriatic fibroblasts: an experimental system for studying the two cell interactions

Francesca Levi-Schaffer^*, Laurent Klapholz, Louis Weinrauch, Meir Shalit

^*Corresponding author for this work

School of Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

In this study we established a coculture system for rat peritoneal mast cells (MC) with psoriatic (PSO) dermal fibroblasts. Rat MC adhered within five minutes to the fibroblasts monolayer and their attachment and viability was maintained for at least 2-3 days. Cocultured MC could be activated to release high percentages of histamine with compound 48 80 indicating that they retained their full functional activity. Attachment, viability and functional activity were similar for MC seeded on PSO fibroblasts or on normal human fibroblasts (NOR) used as a control. This would indicate that PSO fibroblasts altered biochemical characteristics do not interfere with these MC properties. We suggest that this coculture system is a suitable in vitro defined model to study mutual effects of MC and fibroblasts in psoriasis.

Original language	American English
Pages (from-to)	230-234
Number of pages	5
Journal	Journal of the European Academy of Dermatology and Venereology
Volume	4
Issue number	3
DOIs	https://doi.org/10.1016/0926-9959(95)00050-N
State	Published - Jun 1995

Bibliographical note

Funding Information:
Supported in part by a grant from the Joint research Fund of the Hebrew University and Hadassah, and by the Adolf0 and Evelyn Blum Research Fund.

Keywords

Fibroblast
Mast cell
Psoriasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0926-9959(95)00050-N

Cite this

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title = "Coculture of mast cells with psoriatic fibroblasts: an experimental system for studying the two cell interactions",

abstract = "In this study we established a coculture system for rat peritoneal mast cells (MC) with psoriatic (PSO) dermal fibroblasts. Rat MC adhered within five minutes to the fibroblasts monolayer and their attachment and viability was maintained for at least 2-3 days. Cocultured MC could be activated to release high percentages of histamine with compound 48 80 indicating that they retained their full functional activity. Attachment, viability and functional activity were similar for MC seeded on PSO fibroblasts or on normal human fibroblasts (NOR) used as a control. This would indicate that PSO fibroblasts altered biochemical characteristics do not interfere with these MC properties. We suggest that this coculture system is a suitable in vitro defined model to study mutual effects of MC and fibroblasts in psoriasis.",

keywords = "Fibroblast, Mast cell, Psoriasis",

author = "Francesca Levi-Schaffer and Laurent Klapholz and Louis Weinrauch and Meir Shalit",

note = "Funding Information: Supported in part by a grant from the Joint research Fund of the Hebrew University and Hadassah, and by the Adolf0 and Evelyn Blum Research Fund.",

year = "1995",

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doi = "10.1016/0926-9959(95)00050-N",

language = "American English",

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pages = "230--234",

journal = "Journal of the European Academy of Dermatology and Venereology",

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TY - JOUR

T1 - Coculture of mast cells with psoriatic fibroblasts

T2 - an experimental system for studying the two cell interactions

AU - Levi-Schaffer, Francesca

AU - Klapholz, Laurent

AU - Weinrauch, Louis

AU - Shalit, Meir

N1 - Funding Information: Supported in part by a grant from the Joint research Fund of the Hebrew University and Hadassah, and by the Adolf0 and Evelyn Blum Research Fund.

PY - 1995/6

Y1 - 1995/6

N2 - In this study we established a coculture system for rat peritoneal mast cells (MC) with psoriatic (PSO) dermal fibroblasts. Rat MC adhered within five minutes to the fibroblasts monolayer and their attachment and viability was maintained for at least 2-3 days. Cocultured MC could be activated to release high percentages of histamine with compound 48 80 indicating that they retained their full functional activity. Attachment, viability and functional activity were similar for MC seeded on PSO fibroblasts or on normal human fibroblasts (NOR) used as a control. This would indicate that PSO fibroblasts altered biochemical characteristics do not interfere with these MC properties. We suggest that this coculture system is a suitable in vitro defined model to study mutual effects of MC and fibroblasts in psoriasis.

AB - In this study we established a coculture system for rat peritoneal mast cells (MC) with psoriatic (PSO) dermal fibroblasts. Rat MC adhered within five minutes to the fibroblasts monolayer and their attachment and viability was maintained for at least 2-3 days. Cocultured MC could be activated to release high percentages of histamine with compound 48 80 indicating that they retained their full functional activity. Attachment, viability and functional activity were similar for MC seeded on PSO fibroblasts or on normal human fibroblasts (NOR) used as a control. This would indicate that PSO fibroblasts altered biochemical characteristics do not interfere with these MC properties. We suggest that this coculture system is a suitable in vitro defined model to study mutual effects of MC and fibroblasts in psoriasis.

KW - Fibroblast

KW - Mast cell

KW - Psoriasis

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U2 - 10.1016/0926-9959(95)00050-N

DO - 10.1016/0926-9959(95)00050-N

M3 - Article

AN - SCOPUS:0029029585

SN - 0926-9959

VL - 4

SP - 230

EP - 234

JO - Journal of the European Academy of Dermatology and Venereology

JF - Journal of the European Academy of Dermatology and Venereology

IS - 3

ER -

Coculture of mast cells with psoriatic fibroblasts: an experimental system for studying the two cell interactions

Abstract

Bibliographical note

Keywords

UN SDGs

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