Coenzyme A and short-chain Acyl-CoA species in control and ischemic rat brain

Joseph Deutsch, Stanley I. Rapoport*, Thad A. Rosenberger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

A rapid and reliable method was developed to quantify brain concentrations of coenzyme A (CoA) and short-chain acyl-CoAs having chain length ≤ 4 carbon atoms. The method employs tissue extraction and isolation using an oligonucleotide purification cartridge and quantifies concentrations by peak area analysis following high-performance liquid chromatography (HPLC). In adult anesthetized rats subjected to 4-s high-energy microwave irradiation to stop brain metabolism, the brain concentrations of CoA, 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), acetyl-CoA, and butyryl-CoA equaled 68.7 ± 18.5, 2.7 ± 1.5, 7.6 ± 2.3, and 30.6± 15.9 nmol·g-1, respectively. After 5 min of complete ischemia, the brain concentrations of CoA and HMG-CoA increased 2- and 12-fold compared to controls, whereas acetyl-CoA and butyryl-CoA concentrations did not change. Markedly elevated levels of CoA and HMG-CoA following cerebral ischemia may reflect disturbed energy metabolism and altered formation of cholesterol and isoprenoids.

Original languageEnglish
Pages (from-to)1577-1582
Number of pages6
JournalNeurochemical Research
Volume27
Issue number12
DOIs
StatePublished - 1 Dec 2002

Keywords

  • 3-hydroxy-3- methylglutaryl-CoA
  • Acetyl-CoA
  • Acyl-CoA
  • Brain
  • Butyryl-CoA
  • Energy
  • Ischemia
  • Mitochondria
  • Rat

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