TY - JOUR
T1 - Coenzyme A and short-chain Acyl-CoA species in control and ischemic rat brain
AU - Deutsch, Joseph
AU - Rapoport, Stanley I.
AU - Rosenberger, Thad A.
PY - 2002/12/1
Y1 - 2002/12/1
N2 - A rapid and reliable method was developed to quantify brain concentrations of coenzyme A (CoA) and short-chain acyl-CoAs having chain length ≤ 4 carbon atoms. The method employs tissue extraction and isolation using an oligonucleotide purification cartridge and quantifies concentrations by peak area analysis following high-performance liquid chromatography (HPLC). In adult anesthetized rats subjected to 4-s high-energy microwave irradiation to stop brain metabolism, the brain concentrations of CoA, 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), acetyl-CoA, and butyryl-CoA equaled 68.7 ± 18.5, 2.7 ± 1.5, 7.6 ± 2.3, and 30.6± 15.9 nmol·g-1, respectively. After 5 min of complete ischemia, the brain concentrations of CoA and HMG-CoA increased 2- and 12-fold compared to controls, whereas acetyl-CoA and butyryl-CoA concentrations did not change. Markedly elevated levels of CoA and HMG-CoA following cerebral ischemia may reflect disturbed energy metabolism and altered formation of cholesterol and isoprenoids.
AB - A rapid and reliable method was developed to quantify brain concentrations of coenzyme A (CoA) and short-chain acyl-CoAs having chain length ≤ 4 carbon atoms. The method employs tissue extraction and isolation using an oligonucleotide purification cartridge and quantifies concentrations by peak area analysis following high-performance liquid chromatography (HPLC). In adult anesthetized rats subjected to 4-s high-energy microwave irradiation to stop brain metabolism, the brain concentrations of CoA, 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), acetyl-CoA, and butyryl-CoA equaled 68.7 ± 18.5, 2.7 ± 1.5, 7.6 ± 2.3, and 30.6± 15.9 nmol·g-1, respectively. After 5 min of complete ischemia, the brain concentrations of CoA and HMG-CoA increased 2- and 12-fold compared to controls, whereas acetyl-CoA and butyryl-CoA concentrations did not change. Markedly elevated levels of CoA and HMG-CoA following cerebral ischemia may reflect disturbed energy metabolism and altered formation of cholesterol and isoprenoids.
KW - 3-hydroxy-3- methylglutaryl-CoA
KW - Acetyl-CoA
KW - Acyl-CoA
KW - Brain
KW - Butyryl-CoA
KW - Energy
KW - Ischemia
KW - Mitochondria
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=0036912028&partnerID=8YFLogxK
U2 - 10.1023/A:1021614422668
DO - 10.1023/A:1021614422668
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C2 - 12515307
AN - SCOPUS:0036912028
SN - 0364-3190
VL - 27
SP - 1577
EP - 1582
JO - Neurochemical Research
JF - Neurochemical Research
IS - 12
ER -