Combined bacteriophages and antibiotics as an efficient therapy against VRE Enterococcus faecalis in a mouse model

Daniel Gelman, Shaul Beyth, Vanda Lerer, Karen Adler, Ronit Poradosu-Cohen, Shunit Coppenhagen-Glazer, Ronen Hazan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Clinical applications of bacteriophage therapy have been recently gathering significant attention worldwide, used mostly as rescue therapy in cases of near-fatal antibiotic failure. Thus, clinically relevant in-vivo models presenting both short- and long-term implications of phage therapy given as rescue treatment for fulminant infections are of highest importance. In this study, a cocktail consisting of two lytic bacteriophages was used to evaluate the therapeutic efficacy of phage therapy as a rescue treatment for severe septic peritonitis in a mouse model. We established that a single injection of the bacteriophage cocktail was sufficient to completely reverse a 100% mortality trend caused by Vancomycin-Resistant Enterococcus faecalis, with significant improvement in both the clinical state and laboratory test results, and without harmful effects on the microbiome. The combination of bacteriophages with a suboptimal antibiotic regimen imparts an additional beneficial effect on the treatment success.

Original languageAmerican English
Pages (from-to)531-539
Number of pages9
JournalResearch in Microbiology
Volume169
Issue number9
DOIs
StatePublished - Nov 2018

Bibliographical note

Publisher Copyright:
© 2018 Institut Pasteur

Keywords

  • EFDG1
  • EFLK1
  • Mouse peritonitis model
  • Phage therapy
  • Phage-antibiotic combination
  • VRE Enterococcus faecalis

Fingerprint

Dive into the research topics of 'Combined bacteriophages and antibiotics as an efficient therapy against VRE Enterococcus faecalis in a mouse model'. Together they form a unique fingerprint.

Cite this