Combined expression of S-VSPα in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants

Dana Guenoune; Rachel Amir; Hanna Badani; Shmuel Wolf; Shmuel Galili

Combined expression of S-VSPα in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants

Dana Guenoune, Rachel Amir, Hanna Badani, Shmuel Wolf, Shmuel Galili^*

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

Original language	English
Pages (from-to)	1867-1870
Number of pages	4
Journal	Journal of Experimental Botany
Volume	53
Issue number	376
State	Published - Sep 2002

Keywords

Chloroplasts
Gene expression
Improved nutritional quality
Soybean vegetative storage proteins
Total lysine content
Transgenic tobacco plants

Cite this

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title = "Combined expression of S-VSPα in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants",

abstract = "Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.",

keywords = "Chloroplasts, Gene expression, Improved nutritional quality, Soybean vegetative storage proteins, Total lysine content, Transgenic tobacco plants",

author = "Dana Guenoune and Rachel Amir and Hanna Badani and Shmuel Wolf and Shmuel Galili",

year = "2002",

month = sep,

language = "אנגלית",

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AU - Guenoune, Dana

AU - Amir, Rachel

AU - Badani, Hanna

AU - Wolf, Shmuel

AU - Galili, Shmuel

PY - 2002/9

Y1 - 2002/9

N2 - Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

AB - Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

KW - Chloroplasts

KW - Gene expression

KW - Improved nutritional quality

KW - Soybean vegetative storage proteins

KW - Total lysine content

KW - Transgenic tobacco plants

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EP - 1870

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 376

ER -

Combined expression of S-VSPα in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants

Abstract

Keywords

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