Combined expression of S-VSPα in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants

Dana Guenoune, Rachel Amir, Hanna Badani, Shmuel Wolf, Shmuel Galili*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSPα to two different organelles - chloroplasts and vacuoles - in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSPα in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

Original languageAmerican English
Pages (from-to)1867-1870
Number of pages4
JournalJournal of Experimental Botany
Volume53
Issue number376
StatePublished - Sep 2002

Keywords

  • Chloroplasts
  • Gene expression
  • Improved nutritional quality
  • Soybean vegetative storage proteins
  • Total lysine content
  • Transgenic tobacco plants

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