Combining chemistry and topography to fight biofilm formation: Fabrication of micropatterned surfaces with a peptide-based coating

Alona Dolid, Luciana C. Gomes, Filipe J. Mergulhão*, Meital Reches

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


This paper describes the fabrication of antifouling surfaces by the combination of topography and peptide chemistry. The topography of the surface mimics the skin of the shark that can resist biofouling by having a certain microtopography. A peptide-based coating that resists fouling self-assembles on these surfaces. In biofilm formation assays, performed under static conditions, the resulting combination (micropattern with peptide coating) has superior antifouling properties against the Gram-negative and Gram-positive strains tested (Escherichia coli and Staphylococcus epidermidis, respectively) when compared to both micropatterned and peptide-coated surfaces. The same behavior was observed in dynamic assays performed in a parallel plate flow chamber (PPFC) setup, where E. coli could not attach to the micropatterned surface coated with peptide during the 30 min of initial adhesion. These assays, mimicking physiological shear stress conditions, suggest that the peptide-coated surface with micropatterned topography may be promising in reducing adhesion and subsequent biofilm formation in biomedical devices such as urinary catheters and stents, and cardiovascular, dental and orthopedic implants.

Original languageAmerican English
Article number111365
JournalColloids and Surfaces B: Biointerfaces
StatePublished - Dec 2020

Bibliographical note

Funding Information:
M. R. acknowledges the support of the Rosetrees Trust. A. D. would like to thank the H ebrew University Center for Nanoscience and Nanotechnology for their financial support. We would like to thank Ms. Galina Chechelinsky, Dr. Itzik Shweky, and Mr. Maurice Saidian for the help with the fabrication process. We would also like to acknowledge Dr. Vitaly Gutkin for performing the XPS measurements and analysis. This research was also funded by: Base Funding - UIDB/00511/2020 of the L aboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE - funded by national funds through the F CT/MCTES (PIDDAC). L. G. thanks the Portuguese Foundation for Science and Technology (FCT) for the financial support of her work contract through the Scientific Employment Stimulus - Individual Call - [CEECIND/01700/2017]. The authors would like to acknowledge the support from the EU COST Actions ENBA (CA15216) and ENIUS (CA16217).

Publisher Copyright:
© 2020 Elsevier B.V.


  • Biofilm
  • Biofouling
  • Peptide
  • Self-assembly
  • Topography


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