Abstract
Neonates are highly susceptible to infection with enteric pathogens, but the underlying mechanisms are not resolved. We show that neonatal chick colonization with Salmonella enterica serovar Enteritidis requires a virulence-factor-dependent increase in epithelial oxygenation, which drives pathogen expansion by aerobic respiration. Co-infection experiments with an Escherichia coli strain carrying an oxygen-sensitive reporter suggest that S. Enteritidis competes with commensal Enterobacteriaceae for oxygen. A combination of Enterobacteriaceae and spore-forming bacteria, but not colonization with either community alone, confers colonization resistance against S. Enteritidis in neonatal chicks, phenocopying germ-free mice associated with adult chicken microbiota. Combining spore-forming bacteria with a probiotic E. coli isolate protects germ-free mice from pathogen colonization, but the protection is lost when the ability to respire oxygen under micro-aerophilic conditions is genetically ablated in E. coli. These results suggest that commensal Enterobacteriaceae contribute to colonization resistance by competing with S. Enteritidis for oxygen, a resource critical for pathogen expansion.
Original language | American English |
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Pages (from-to) | 128-139.e5 |
Journal | Cell Host and Microbe |
Volume | 25 |
Issue number | 1 |
DOIs | |
State | Published - 9 Jan 2019 |
Externally published | Yes |
Bibliographical note
Funding Information:Y.L. was supported by Vaadia-BARD Postdoctoral Fellowship FI-505-2014. Work in H.Z.'s laboratory is supported by the California Agricultural Experimental Station and USDA/NIFA Multistate Research Project NE1334. This work was supported by USDA/NIFA award 2015-67015-22930 (A.J. Bäumler and H.Z.) and by Public Health Service Grants AI060555 (E.M.V. and G.T.W.), AI044170 (A.J. Bäumler), AI096528 (A.J. Bäumler), AI112445 (A.J. Bäumler), and AI112949 (A.J. Bäumler and R.M.T.).
Funding Information:
Y.L. was supported by Vaadia-BARD Postdoctoral Fellowship FI-505-2014. Work in H.Z.?s laboratory is supported by the California Agricultural Experimental Station and USDA/NIFA Multistate Research Project NE1334. This work was supported by USDA/NIFA award 2015-67015-22930 (A.J. B?umler and H.Z.) and by Public Health Service Grants AI060555 (E.M.V. and G.T.W.), AI044170 (A.J. B?umler), AI096528 (A.J. B?umler), AI112445 (A.J. B?umler), and AI112949 (A.J. B?umler and R.M.T.).
Funding Information:
Y.L. was supported by Vaadia-BARD Postdoctoral Fellowship FI-505-2014. Work in H.Z.’s laboratory is supported by the California Agricultural Experimental Station and USDA /NIFA Multistate Research Project NE1334. This work was supported by USDA/NIFA award 2015-67015-22930 (A.J. Bäumler and H.Z.) and by Public Health Service Grants AI060555 (E.M.V. and G.T.W.), AI044170 (A.J. Bäumler), AI096528 (A.J. Bäumler), AI112445 (A.J. Bäumler), and AI112949 (A.J. Bäumler and R.M.T.).
Publisher Copyright:
© 2018 Elsevier Inc.
Keywords
- Enterobacteriaceae
- Salmonella
- colonization resistance
- microbiota
- neonate
- oxygen