TY - JOUR
T1 - Comparative assessment of two extraction procedures for determination of bioactive compounds in some berries used for daily food consumption
AU - Namiesnik, Jacek
AU - Vearasilp, Kann
AU - Leontowicz, Hanna
AU - Leontowicz, Maria
AU - Ham, Kyung Sik
AU - Kang, Seong Gook
AU - Park, Yang Kyun
AU - Arancibia-Avila, Patricia
AU - Toledo, Fernando
AU - Gorinstein, Shela
PY - 2014/2
Y1 - 2014/2
N2 - Two extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol extracts in gooseberries, blueberries and cranberries differed, but not always significantly. Water extracts of gooseberries showed the lowest amounts of polyphenols (mg GAE g-1), 6.24 ± 0.6, and flavonoids (mg CE g-1), 0.29 ± 0.01, and AAs (μMTE g-1) determined by DPPH, FRAP, ABTS and CUPRAC assays such as 6.05 ± 0.6, 8.07 ± 0.9, 18.70 ± 1.8 and 13.44 ± 1.2, respectively, in comparison with blueberries and cranberries. Polyphenol content highly correlated with antioxidant activity (R2 from 0.94 to 0.81). The quenching properties of berries were studied by the interaction of water and methanol polyphenol extracts with HSA by 3D fluorescence. In conclusion, the bioactivity of gooseberries was lower than in blueberries and cranberries. Gooseberries can be used as a new source for food consumption and supplementation based on their antioxidant and binding properties. 3D fluorescence spectroscopy and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of different food products.
AB - Two extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol extracts in gooseberries, blueberries and cranberries differed, but not always significantly. Water extracts of gooseberries showed the lowest amounts of polyphenols (mg GAE g-1), 6.24 ± 0.6, and flavonoids (mg CE g-1), 0.29 ± 0.01, and AAs (μMTE g-1) determined by DPPH, FRAP, ABTS and CUPRAC assays such as 6.05 ± 0.6, 8.07 ± 0.9, 18.70 ± 1.8 and 13.44 ± 1.2, respectively, in comparison with blueberries and cranberries. Polyphenol content highly correlated with antioxidant activity (R2 from 0.94 to 0.81). The quenching properties of berries were studied by the interaction of water and methanol polyphenol extracts with HSA by 3D fluorescence. In conclusion, the bioactivity of gooseberries was lower than in blueberries and cranberries. Gooseberries can be used as a new source for food consumption and supplementation based on their antioxidant and binding properties. 3D fluorescence spectroscopy and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of different food products.
KW - Antioxidant activity
KW - Berries
KW - Bioactive compounds
KW - Food consumption
UR - http://www.scopus.com/inward/record.url?scp=84892477532&partnerID=8YFLogxK
U2 - 10.1111/ijfs.12287
DO - 10.1111/ijfs.12287
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AN - SCOPUS:84892477532
SN - 0950-5423
VL - 49
SP - 337
EP - 346
JO - International Journal of Food Science and Technology
JF - International Journal of Food Science and Technology
IS - 2
ER -