Comparison between different assays for superoxide dismutase-like activity

Sara Goldstein*, Gidon Czapski

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The direct and indirect methods for assaying the superoxide dismutase activity of a compound are compared. With the use of a direct method. the mechanism of the catalysis of O2-dismutation by the tested compound can be determined. while with the indirect method it cannot. and this may lead to misinterpretation of the results. Assuming that the catalysis occurs via the 'ping-pong' mechanism, both the direct and indirect methods are limited to the determination of values of kcat ≥ 105M-1s-1 and kcat ≥ 3 × 106M-1s-1. respectively. Moreover, many side reactions may occur with the indirect method which may interfere with the measurements. Nevertheless. the indirect method approximates better the in vivo conditions than the direct method, and a tested compound that has high SOD activity using a direct method and low SOD activity using an indirect method. will most probably be a poor SOD mimic in vivo.

Original languageEnglish
Pages (from-to)5-10
Number of pages6
JournalFree Radical Research
Volume12
Issue number1
DOIs
StatePublished - 1991

Keywords

  • Assay
  • Catalysis
  • Dismutation
  • SOD

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