TY - JOUR
T1 - Comparison of dimethyl dioctadecyl ammonium bromide, Freund's complete adjuvant and mineral oil for induction of humoral antibodies, cellular immunity and resistance to Newcastle disease virus in chickens
AU - Katz, David
AU - Inbar, Itzhak
AU - Samina, Itzhak
AU - Peleg, Ben Ami
AU - Heller, Dan E.
PY - 1993/12
Y1 - 1993/12
N2 - Dimethyl diotadecyl ammonium bromide (DDA), a lipophilic quaternary amine, was evaluated in adult chickens for potentiation of immunological responses to subcutaneously administered inactivated Newcastle disease virus (NDV) vaccines. DDA enhanced humoral and cell-mediated immune (CMI) responses to levels which were significantly higher than those induced by the vaccine alone The haemagglutination inhibition antibody titers induced by DDA were slightly lower than those induced by mineral oil although neutralizing antibody titers seemed to be higher. DDA induced strong CMI (DTH and lymphocyte proliferation) responses, more than those induced by Freund's complete adjuvant and mineral oil. Both DDA and mineral oil induced comparable high levels of protection to challenge doses of 200 000 LD50 per chicken. No toxic effects or local tissue damage were observed in any of the inoculated chickens.
AB - Dimethyl diotadecyl ammonium bromide (DDA), a lipophilic quaternary amine, was evaluated in adult chickens for potentiation of immunological responses to subcutaneously administered inactivated Newcastle disease virus (NDV) vaccines. DDA enhanced humoral and cell-mediated immune (CMI) responses to levels which were significantly higher than those induced by the vaccine alone The haemagglutination inhibition antibody titers induced by DDA were slightly lower than those induced by mineral oil although neutralizing antibody titers seemed to be higher. DDA induced strong CMI (DTH and lymphocyte proliferation) responses, more than those induced by Freund's complete adjuvant and mineral oil. Both DDA and mineral oil induced comparable high levels of protection to challenge doses of 200 000 LD50 per chicken. No toxic effects or local tissue damage were observed in any of the inoculated chickens.
KW - Antibody
KW - Cell-mediated immunity
KW - Dimethyl dioctadecyl ammonium bromide
KW - Freund's complete adjuvant
KW - Immunological adjuvants
KW - Mineral oil
KW - Newcastle disease virus
KW - Protection
UR - http://www.scopus.com/inward/record.url?scp=0027752993&partnerID=8YFLogxK
U2 - 10.1016/0928-8244(93)90051-5
DO - 10.1016/0928-8244(93)90051-5
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C2 - 8136781
AN - SCOPUS:0027752993
SN - 0928-8244
VL - 7
SP - 303
EP - 313
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 4
ER -