TY - JOUR
T1 - Competitive hybridization
T2 - Theory and application in isolation and quantification of differentially regulated genes
AU - Zhong, Guang Yan
AU - Riov, Joseph
AU - Goren, Raphael
AU - Holland, Doron
PY - 2000/6/15
Y1 - 2000/6/15
N2 - Competitive hybridization is a simple yet powerful method that was developed to screen cDNA libraries for differentially regulated genes. The method is based on competition between unlabeled cDNA from the mRNA of one sample and labeled cDNA from another sample. By manipulating the amount of competing unlabeled cDNA, background signals from the nonregulated genes can be increased or reduced, enabling the signals from differentially regulated genes to be contrasted and to be identified in a quantitative manner. To demonstrate the feasibility of the method, we screened a citrus cDNA library for ethylene-induced genes and identified three genes with different levels of ethylene induction. The mathematical basis of the method and its possible application in gene chip technology are discussed. (C) 2000 Academic Press.
AB - Competitive hybridization is a simple yet powerful method that was developed to screen cDNA libraries for differentially regulated genes. The method is based on competition between unlabeled cDNA from the mRNA of one sample and labeled cDNA from another sample. By manipulating the amount of competing unlabeled cDNA, background signals from the nonregulated genes can be increased or reduced, enabling the signals from differentially regulated genes to be contrasted and to be identified in a quantitative manner. To demonstrate the feasibility of the method, we screened a citrus cDNA library for ethylene-induced genes and identified three genes with different levels of ethylene induction. The mathematical basis of the method and its possible application in gene chip technology are discussed. (C) 2000 Academic Press.
KW - Competitive hybridization
KW - Differential gene screening
KW - Ethylene-induced genes
UR - http://www.scopus.com/inward/record.url?scp=0342647296&partnerID=8YFLogxK
U2 - 10.1006/abio.2000.4581
DO - 10.1006/abio.2000.4581
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C2 - 10860509
AN - SCOPUS:0342647296
SN - 0003-2697
VL - 282
SP - 129
EP - 135
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -