Competitive hybridization: Theory and application in isolation and quantification of differentially regulated genes

Guang Yan Zhong, Joseph Riov, Raphael Goren, Doron Holland*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Competitive hybridization is a simple yet powerful method that was developed to screen cDNA libraries for differentially regulated genes. The method is based on competition between unlabeled cDNA from the mRNA of one sample and labeled cDNA from another sample. By manipulating the amount of competing unlabeled cDNA, background signals from the nonregulated genes can be increased or reduced, enabling the signals from differentially regulated genes to be contrasted and to be identified in a quantitative manner. To demonstrate the feasibility of the method, we screened a citrus cDNA library for ethylene-induced genes and identified three genes with different levels of ethylene induction. The mathematical basis of the method and its possible application in gene chip technology are discussed. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)129-135
Number of pages7
JournalAnalytical Biochemistry
Volume282
Issue number1
DOIs
StatePublished - 15 Jun 2000

Keywords

  • Competitive hybridization
  • Differential gene screening
  • Ethylene-induced genes

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