TY - JOUR
T1 - Concatenated logic gates using four coupled biocatalysts operating in series
AU - Niazov, Tamara
AU - Baron, Ronan
AU - Katz, Eugenii
AU - Lioubashevski, Oleg
AU - Willner, Itamar
PY - 2006/11/14
Y1 - 2006/11/14
N2 - The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.
AB - The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.
KW - Biocatalysis
KW - Biocomputers
KW - Enzymes
UR - http://www.scopus.com/inward/record.url?scp=33751229022&partnerID=8YFLogxK
U2 - 10.1073/pnas.0608319103
DO - 10.1073/pnas.0608319103
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C2 - 17088533
AN - SCOPUS:33751229022
SN - 0027-8424
VL - 103
SP - 17160
EP - 17163
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 46
ER -