Concatenated logic gates using four coupled biocatalysts operating in series

Tamara Niazov; Ronan Baron; Eugenii Katz; Oleg Lioubashevski; Itamar Willner

doi:10.1073/pnas.0608319103

Concatenated logic gates using four coupled biocatalysts operating in series

Tamara Niazov
, Ronan Baron
, Eugenii Katz
, Oleg Lioubashevski
, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

186 Scopus citations

Abstract

The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD⁺-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O₂, and glucose are used to drive the concatenated-gates system. The cofactor NAD⁺, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.

Original language	English
Pages (from-to)	17160-17163
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	46
DOIs	https://doi.org/10.1073/pnas.0608319103
State	Published - 14 Nov 2006

Keywords

Biocatalysis
Biocomputers
Enzymes

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abstract = "The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.",

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AU - Baron, Ronan

AU - Katz, Eugenii

AU - Lioubashevski, Oleg

AU - Willner, Itamar

PY - 2006/11/14

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N2 - The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.

AB - The assembly of three concatenated enzyme-based logic gates consisting of OR, AND, XOR is described. Four biocatalysts, acetylcholine esterase, choline oxidase, microperoxidase-11, and the NAD+-dependent glucose dehydrogenase, are used to assemble the gates. Four inputs that include acetylcholine, butyrylcholine, O2, and glucose are used to drive the concatenated-gates system. The cofactor NAD+, and its reduced 1,4-dihydro form, NADH, are used as a reporter couple, and these provide an optical output for the gates. The modulus of the absorbance changes of NADH is used as a readout signal.

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KW - Biocomputers

KW - Enzymes

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Concatenated logic gates using four coupled biocatalysts operating in series

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Keywords

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