Concentration- and temperature-induced effects of incorporated desmopressin on the properties of reverse hexagonal mesophase

Dima Libster, Abraham Aserin, Doron Yariv, Gil Shoham, Nissim Garti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


In this paper we report on the solubilization of desmopressin, as a model for peptide drugs, into reverse hexagonal (HII) liquid crystals. Concentration- and temperature-induced interactions of desmopressin, as well as the conformation of the peptide, were studied using small-angle X-ray scattering, ATR-FTIR spectroscopy, SD-NMR, and rheological measurements. A considerable increase (up to 6 Å) in the lattice parameter of the mesophases was obtained upon incorporation of the peptide. According to the ATR-FTIR analysis, the chaotropic effect of peptide embedment was assigned to its interactions with hydroxyls of monoglyceride in the outer interface region. These interactions had only a minor influence on the conformation of the peptide; weakening or opening the γ-turns resulted in partial binding of the peptide's free carbonyls to monoolein. Temperature-dependent SAXS measurements displayed a chaotropic destabilizing effect of desmopressin on the structure, shifting toward the lower temperature HII-L 2structural transition. Temperature increase resulted in an increase of the domain size in the presence of the peptide, in contrast to the trend observed in the empty mesophase. SD-NMR analysis enabled distinguishing between two factors impeding the diffusion of the peptide: the restriction of motion due to the geometrical constrain of diffusion within the water tubes, and the interactions of the guest molecule with monoglyceride. The onset of the critical behavior at 45 °C was found to be significant, indicating considerable weakening of the monoglyceride and desmopressin interactions and the destabilizing effect of the peptide on the mesophase above this temperature. Similar temperature-dependent behavior was revealed by rheological measurements displaying the same onset of the critical behavior. It was demonstrated by Franz diffusion cell measurements that hexagonal mesophases can potentially be used as delivery vehicles for sustained delivery of desmopressin.

Original languageAmerican English
Pages (from-to)6336-6346
Number of pages11
JournalJournal of Physical Chemistry B
Issue number18
StatePublished - 7 May 2009


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