TY - JOUR
T1 - Concentration-based self-assembly of phycocyanin
AU - Eisenberg, Ido
AU - Harris, Dvir
AU - Levi-Kalisman, Yael
AU - Yochelis, Shira
AU - Shemesh, Asaf
AU - Ben-Nissan, Gili
AU - Sharon, Michal
AU - Raviv, Uri
AU - Adir, Noam
AU - Keren, Nir
AU - Paltiel, Yossi
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media Dordrecht.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.
AB - Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.
KW - Native mass spectrometry
KW - Oligomerization
KW - Phycocyanin
KW - SAXS
KW - TEM
UR - http://www.scopus.com/inward/record.url?scp=85020124585&partnerID=8YFLogxK
U2 - 10.1007/s11120-017-0406-7
DO - 10.1007/s11120-017-0406-7
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C2 - 28577216
AN - SCOPUS:85020124585
SN - 0166-8595
VL - 134
SP - 39
EP - 49
JO - Photosynthesis Research
JF - Photosynthesis Research
IS - 1
ER -