Concentration-based self-assembly of phycocyanin

Ido Eisenberg; Dvir Harris; Yael Levi-Kalisman; Shira Yochelis; Asaf Shemesh; Gili Ben-Nissan; Michal Sharon; Uri Raviv; Noam Adir; Nir Keren; Yossi Paltiel

doi:10.1007/s11120-017-0406-7

Concentration-based self-assembly of phycocyanin

Ido Eisenberg, Dvir Harris, Yael Levi-Kalisman, Shira Yochelis, Asaf Shemesh, Gili Ben-Nissan, Michal Sharon, Uri Raviv, Noam Adir, Nir Keren, Yossi Paltiel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.

Original language	American English
Pages (from-to)	39-49
Number of pages	11
Journal	Photosynthesis Research
Volume	134
Issue number	1
DOIs	https://doi.org/10.1007/s11120-017-0406-7
State	Published - 1 Oct 2017

Bibliographical note

Funding Information:
This research was supported by a Grant No. 3-12405(151235) from the Ministry of Science, Technology and Space, Israel, the National Science Council (NSC) of Taiwan, the Israel Science Foundation (843/16) and the US-Israel Bi-National Science Fund (2014395).

Publisher Copyright:
© 2017, Springer Science+Business Media Dordrecht.

Keywords

Native mass spectrometry
Oligomerization
Phycocyanin
SAXS
TEM

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10.1007/s11120-017-0406-7

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abstract = "Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.",

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AU - Ben-Nissan, Gili

AU - Sharon, Michal

AU - Raviv, Uri

AU - Adir, Noam

AU - Keren, Nir

AU - Paltiel, Yossi

N1 - Funding Information: This research was supported by a Grant No. 3-12405(151235) from the Ministry of Science, Technology and Space, Israel, the National Science Council (NSC) of Taiwan, the Israel Science Foundation (843/16) and the US-Israel Bi-National Science Fund (2014395). Publisher Copyright: © 2017, Springer Science+Business Media Dordrecht.

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N2 - Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.

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Concentration-based self-assembly of phycocyanin

Abstract

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Keywords

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