Abstract
Biochemical events occurring under restrictive and permissive conditions of infection with the temperature-sensitive mutant ts 75 (strain B77 avian sarcoma virus) were studied. This virus is able to carry out both DNA and DNA-dependent RNA synthesis during incubation at the nonpermissive temperature. After shift to the permissive temperature these synthetic activities are no longer required for maturation of infectious virus. However, continuous protein synthesis is needed after the shift to permissive conditions to allow maturation of infectious virus. Analysis of virion polypeptides by polyacrylamide gel electrophoresis revealed differences in the proportion of polypeptides labeled at the restrictive as compared to the permissive temperature. At least one of them (polypeptide 1) is produced only in low quantities at the restrictive temperature but is made and incorporated into virus particles very extensively after the shift to the permissive temperature.
Original language | English |
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Pages (from-to) | 745-753 |
Number of pages | 9 |
Journal | Virology |
Volume | 46 |
Issue number | 3 |
DOIs | |
State | Published - Dec 1971 |
Externally published | Yes |