Confinement to Organelle-Associated Inclusion Structures Mediates Asymmetric Inheritance of Aggregated Protein in Budding Yeast

Rachel Spokoini, Ofer Moldavski, Yaakov Nahmias, Jeremy L. England, Maya Schuldiner, Daniel Kaganovich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

155 Scopus citations

Abstract

The division of the S. cerevisiae budding yeast, which produces one mother cell and one daughter cell, is asymmetric with respect to aging. Remarkably, the asymmetry of yeast aging coincides with asymmetric inheritance of damaged and aggregated proteins by the mother cell. Here, we show that misfolded proteins are retained in the mother cell by being sequestered in juxtanuclear quality control compartment (JUNQ) and insoluble protein deposit (IPOD) inclusions, which are attached to organelles. Upon exposure to stress, misfolded proteins accumulate in stress foci that must be disaggregated by Hsp104 in order to be degraded or processed to JUNQ and IPOD. Cells that fail to deliver aggregates to an inclusion pass on aggregates to subsequent generations. The ability of cells to asymmetrically segregate aggregates during division has become an increasingly fascinating topic in the field of aggregation and aging. In this study, Kaganovich and colleagues advance our cellular understanding of aging by showing that confinement of aggregated proteins is precisely regulated by compartmentalization in inclusions and is key to preventing generational transfer of aggregated proteins. The authors provide mechanistic insight that enables control of asymmetric aggregate inheritance.

Original languageAmerican English
Pages (from-to)738-747
Number of pages10
JournalCell Reports
Volume2
Issue number4
DOIs
StatePublished - 25 Oct 2012

Bibliographical note

Funding Information:
We thank members of the Kaganovich lab and Margaret Cunniff for valuable discussion of experiments and comments on the manuscript and Meytal Waiss for technical assistance. D.K. and R.S. are supported by an Israel Science Foundation grant (ISF 843/11), a grant from the Abisch-Frenkel Foundation, and a grant from the GIF, the German-Israeli Foundation for Scientific Research and Development (2267-2166.9/2010). M.S. and O.M. are supported by an ERC-StG-2010 260395 - ER Architecture grant.

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