TY - JOUR
T1 - Confirmation of the Presence of Mycobacterium tuberculosis Complex-Specific DNA in Three Archaeological Specimens
AU - Spigelman, Mark
AU - Matheson, Carney
AU - Lev, Galit
AU - Greenblatt, Charles
AU - Donoghue, Helen D.
PY - 2002/11
Y1 - 2002/11
N2 - This journal published the first reported identification of Mycobacterium tuberculosis complex (MTB) DNA in ancient human remains but concerns were raised about the article two years after publication. These were based on methodology which, in the field of ancient DNA, was still developing. Here we present a re-examination of the 1993 research conducted on three specimens which exhibited palaeopathologies indicative of tuberculosis. The specimens were: an ulna from pre-European-contact Borneo, a spine from Byzantine Turkey, and a lumbar-sacral spine from 17th century Scotland. There was insufficient material to permit re-examination of all of the original samples. The earlier results were confirmed in two independent laboratories using different methodologies. MTB DNA complex-specific DNA amplicons were obtained, and sequenced in both laboratories, in a re-analysis of samples which supported the earlier findings.
AB - This journal published the first reported identification of Mycobacterium tuberculosis complex (MTB) DNA in ancient human remains but concerns were raised about the article two years after publication. These were based on methodology which, in the field of ancient DNA, was still developing. Here we present a re-examination of the 1993 research conducted on three specimens which exhibited palaeopathologies indicative of tuberculosis. The specimens were: an ulna from pre-European-contact Borneo, a spine from Byzantine Turkey, and a lumbar-sacral spine from 17th century Scotland. There was insufficient material to permit re-examination of all of the original samples. The earlier results were confirmed in two independent laboratories using different methodologies. MTB DNA complex-specific DNA amplicons were obtained, and sequenced in both laboratories, in a re-analysis of samples which supported the earlier findings.
KW - Ancient DNA
KW - Mycobacterium tuberculosis
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=0346976513&partnerID=8YFLogxK
U2 - 10.1002/oa.638
DO - 10.1002/oa.638
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AN - SCOPUS:0346976513
SN - 1047-482X
VL - 12
SP - 393
EP - 401
JO - International Journal of Osteoarchaeology
JF - International Journal of Osteoarchaeology
IS - 6
ER -
